Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou, Hainan, China; College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, China.
College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, China.
Fish Shellfish Immunol. 2020 Oct;105:359-368. doi: 10.1016/j.fsi.2020.06.058. Epub 2020 Jul 18.
Quercetin, a potential fish food supplement, has been reported to process many beneficial properties. However, some negative effects of quercetin have been observed, which pointed out necessity for additional studies to evaluate its safety. Therefore, the present study investigated effects of quercetin (0.01, 0.1, 1, 10, 100 and 1000 μg/L) on shoaling and anxiety behaviors through novel tank tests in zebrafish (Danio rerio). Furthermore, oxidative stress, neuroinflammation and apoptosis in the brains were examined to learn more about mechanisms of action related to quercetin. The results showed that quercetin at the lower concentrations exerted beneficial effects on shoaling and anxiety behaviors. On the contrary, when quercetin was up to 1000 μg/L, it exerted detrimental effects shown as decreases of movement and increases of anxiety behaviors. Generally, U-shaped responses of antioxidant enzyme activities (superoxide dismutase and catalase), and inversed U-shaped responses of inflammatory mediators (cyclooxygenase-2) and cytokines (interleukin-1β, interleukin-6, interleukin-10, and tumor necrosis factor α) to quercetin treatment were found in the brains. In addition, quercetin at the lower concentrations attenuated cell apoptosis, while even more apoptosis was found at the 1000 μg/L quercetin group. In conclusion, quercetin could exert beneficial or detrimental effects on the shoaling and anxiety behaviors depending on the treatment concentrations, and the underlying mechanisms are potentially associated with neuroinflammation and neuron apoptosis.
槲皮素是一种有潜力的鱼类饲料补充剂，据报道具有许多有益特性。然而，已经观察到槲皮素的一些负面作用，这指出需要进行额外的研究来评估其安全性。因此，本研究通过斑马鱼（Danio rerio）的新鱼缸测试，研究了槲皮素（0.01、0.1、1、10、100 和 1000μg/L）对群居和焦虑行为的影响。此外，还检查了大脑中的氧化应激、神经炎症和细胞凋亡，以更深入地了解与槲皮素相关的作用机制。结果表明，低浓度的槲皮素对群居和焦虑行为有有益的影响。相反，当槲皮素达到 1000μg/L 时，它会产生不利的影响，表现为运动减少和焦虑行为增加。总的来说，在大脑中发现抗氧化酶活性（超氧化物歧化酶和过氧化氢酶）呈 U 形反应，炎症介质（环加氧酶-2）和细胞因子（白细胞介素-1β、白细胞介素-6、白细胞介素-10 和肿瘤坏死因子α）呈倒 U 形反应，对槲皮素处理的反应。此外，低浓度的槲皮素可减轻细胞凋亡，而在 1000μg/L 槲皮素组中则发现更多的凋亡。总之，槲皮素对群居和焦虑行为可能产生有益或有害的影响，这取决于治疗浓度，其潜在机制可能与神经炎症和神经元凋亡有关。
