Department of Oncology and Gerontology, The First Affiliated Hospital, Xi'an Medical University, 48 Fenghao West Road, Xi'an, 710077, China.
School of Clinical Medicine, Xi'an Medical University, Xi'an, China.
Sci Rep. 2020 Jul 21;10(1):12092. doi: 10.1038/s41598-020-69000-3.
This study was to investigate the association between serum interleukin 32 (IL-32) concentration and clinical parameters in patients with stable chronic obstructive pulmonary disease (COPD). One hundred and sixteen patients with stable COPD and 70 healthy subjects were included in the study. The serum concentration of IL-32 was detected by enzyme-linked immunosorbent assay. The correlation between serum IL-32 and clinical parameters of patients with COPD was analyzed by T-test, one-way analysis of variance, multiple linear regression and receiver operating characteristic curve. The serum concentration of IL-32 in patients with stable COPD was higher than that in healthy control group (p < 0.001) and increased serum IL-32 was positively correlated with GOLD grading (p = 0.026), mMRC score (p = 0.004) and clinical medical history (p = 0.005), but negatively related to FEV1/FVC (p = 0.001) and FEV1% predicted (p = 0.001). Patient's COPD grading (p = 0.001), clinical medical history (p < 0.001) and FEV1/FVC (p = 0.001) exerted a significant impact on serum IL-32. The sensitivity and specificity of serum IL-32 for discerning COPD patients from healthy individuals were 85.34% and 64.29%, and the area under the curve was 0.808 (p < 0.001). Increased IL-32 is involved in the chronic disease progression of COPD, suggesting that IL-32 may be a molecular biomarker that reflects the severity of COPD and contributes to the disease diagnosis.
本研究旨在探讨血清白细胞介素 32（IL-32）浓度与稳定期慢性阻塞性肺疾病（COPD）患者临床参数之间的关系。纳入 116 例稳定期 COPD 患者和 70 例健康对照者。采用酶联免疫吸附试验检测血清 IL-32 浓度。采用 T 检验、单因素方差分析、多元线性回归和受试者工作特征曲线分析 COPD 患者血清 IL-32 与临床参数的相关性。稳定期 COPD 患者血清 IL-32 浓度高于健康对照组（p<0.001），且升高的血清 IL-32 与 GOLD 分级（p=0.026）、mMRC 评分（p=0.004）和临床病史（p=0.005）呈正相关，与 FEV1/FVC（p=0.001）和 FEV1%预计值（p=0.001）呈负相关。患者 COPD 分级（p=0.001）、临床病史（p<0.001）和 FEV1/FVC（p=0.001）对血清 IL-32 有显著影响。血清 IL-32 鉴别 COPD 患者与健康个体的敏感性和特异性分别为 85.34%和 64.29%，曲线下面积为 0.808（p<0.001）。升高的 IL-32 参与 COPD 的慢性疾病进展，提示 IL-32 可能是反映 COPD 严重程度的分子生物标志物，有助于疾病诊断。
