He Yu, Wang Junping, Wang Shuo
State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China.
Medical College, Nankai University, Tianjin 300350, China.
Int J Anal Chem. 2020 Jul 10;2020:5819062. doi: 10.1155/2020/5819062. eCollection 2020.
This work demonstrates rapid sensing of kaempferol using active sensing material synthesized using the one-pot surface-imprinting synthesis method. This sensor consisted of molecularly imprinted polymer (MIP) consisting of mesoporous molecular sieves (SBA-15) loaded with carbon dots (CDs). Fourier transform infrared (FT-IR) spectroscopy confirmed successful incorporation of CDs onto the surface of imprinted mesoporous molecular sieves. Ordered hexagonal arrays of CDs@SBA-15@MIP mesopore structure were confirmed with transmission electron microscopy. Fluorescence intensity of CDs@SBA-15@MIP composites linearly correlated with kaempferol content in the 0.05-2 mg/L range. Detection limit was 14 g/L. MIPs were used for efficient detection of kaempferol in fruit and vegetable samples with recovery values from 80% to 112%. The method has high sensitivity, low cost, good selectivity, and many application potentials useful for research and development of flavonoid monomer presence in food.
这项工作展示了使用通过一锅法表面印迹合成法合成的活性传感材料对山奈酚的快速传感。该传感器由负载有碳点（CDs）的介孔分子筛（SBA - 15）组成的分子印迹聚合物（MIP）构成。傅里叶变换红外（FT - IR）光谱证实了碳点成功掺入印迹介孔分子筛表面。通过透射电子显微镜确认了CDs@SBA - 15@MIP介孔结构的有序六边形阵列。CDs@SBA - 15@MIP复合材料的荧光强度在0.05 - 2 mg/L范围内与山奈酚含量呈线性相关。检测限为14 μg/L。分子印迹聚合物用于高效检测水果和蔬菜样品中的山奈酚，回收率在80%至112%之间。该方法具有高灵敏度、低成本、良好的选择性，并且在食品中黄酮类单体存在的研究和开发方面具有许多应用潜力。
