Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, PR China.
College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, PR China.
Biosens Bioelectron. 2016 Mar 15;77:950-6. doi: 10.1016/j.bios.2015.10.072. Epub 2015 Oct 27.
A novel sensitive fluorescent sensor for determination of sterigmatocystin (ST), which was based on carbon dots-embedded molecularly imprinted polymer (CDs@MIP), was prepared by an efficient one-pot reaction. First, highly blue luminescent CDs were synthesized via a one-step reaction. Then, through a non-hydrolytic sol-gel process, MIP was formed on the CDs surface in the presence of 1,8-dihydroxyanthraquinone as an alternative template molecule to obtain CDs@MIP. The CDs acted as antennas for signal amplification and optical readout, and the MIP coated on the CDs surface provided specific binding sites for ST. The performance of CDs@MIP was compared with that of CDs embedded in non-imprinted polymer (CDs@NIP). CDs@MIP exhibited high selectivity and sensitivity toward ST. Under optimized conditions, the relative fluorescence intensity of CDs@MIP decreased linearly with the concentration of ST from 0.05 to 2.0 mgL(-1) with a detection limit of 0.019 mgL(-1) (S/N=3) and the precision for five replicate detections of 0.10 mgL(-1) ST was 2.31%. The sensor was also used to determine the content of ST in grains with satisfactory results.
一种基于碳点嵌入分子印迹聚合物（CDs@MIP）的新型灵敏荧光传感器，用于测定桔青霉素（ST），通过高效的一锅反应制备。首先，通过一步反应合成了具有高蓝色发光的 CDs。然后，通过非水解溶胶-凝胶过程，在 1,8-二羟基蒽醌作为替代模板分子的存在下，在 CDs 表面形成 MIP，以获得 CDs@MIP。CDs 作为信号放大和光学读出的天线，而涂覆在 CDs 表面的 MIP 为 ST 提供了特定的结合位点。将 CDs@MIP 的性能与嵌入非印迹聚合物的 CDs（CDs@NIP）进行了比较。CDs@MIP 对 ST 表现出高选择性和灵敏度。在优化条件下，CDs@MIP 的相对荧光强度随 ST 浓度从 0.05 至 2.0 mgL(-1)线性降低，检测限为 0.019 mgL(-1)（S/N=3），0.10 mgL(-1) ST 的五次重复检测的精密度为 2.31%。该传感器还用于测定谷物中 ST 的含量，结果令人满意。
