Sun Peng, Tong Juhua, Li Xianen
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.
Int J Anal Chem. 2020 Jul 6;2020:5974130. doi: 10.1155/2020/5974130. eCollection 2020.
Nowadays, there is a growing concern about the quality of herbs used in traditional Chinese medicine. In this study, we evaluated the impacts of paclobutrazol and cultivation period on steroid saponins in . A rapid method to simultaneously determine three principle steroid saponins (ophiopogonins B, D, and D') using ultraperformance liquid chromatography combined with an evaporative light-scattering detector was developed. The contents of three saponins in paclobutrazol-treated and nontreated Sichuan and those in the 2-year and 3-year Zhejiang were analyzed. The results showed that the saponin contents were sharply reduced in paclobutrazol-treated as compared to the control, whereas the concentrations of the three targeted saponins in Zhejiang varied with the increase in cultivation years, reflecting varied effects on saponins. Our study provided chemical evidences for further quality control and agricultural practices of .
如今，人们对中药中使用的草药质量越来越关注。在本研究中，我们评估了多效唑和种植期对[植物名称]中甾体皂苷的影响。开发了一种使用超高效液相色谱结合蒸发光散射检测器同时测定三种主要甾体皂苷（麦冬皂苷B、D和D'）的快速方法。分析了多效唑处理和未处理的四川[植物名称]中三种皂苷的含量以及两年生和三年生浙江[植物名称]中三种皂苷的含量。结果表明，与对照相比，多效唑处理的[植物名称]中皂苷含量急剧降低，而浙江[植物名称]中三种目标皂苷的浓度随种植年份的增加而变化，反映了对皂苷的不同影响。我们的研究为[植物名称]的进一步质量控制和农业实践提供了化学证据。
