College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Molecules. 2019 Sep 4;24(18):3220. doi: 10.3390/molecules24183220.
Ophiopogonis Radix, also known as Mai-dong in Chinese, was a commonly used traditional Chinese medicine (TCM) and functional health food. Two products of Ophiopogonis Radix are largely produced in the Sichuan and Zhejiang province, which are called "Chuan maidong (CMD)" and "Zhe maidong (ZMD)" respectively. To distinguish and evaluate the quality of CMD and ZMD, an analytical method based on ultra-fast performance liquid chromatography coupled with triple quadrupole-linear ion trap mass spectrometry (UFLC-QTRAP-MS/MS) was established for simultaneous determination of 32 constituents including 4 steroidal saponins, 3 homisoflavonoids, 15 amino acids, and 10 nucleosides in 27 Mai-dong samples from Sichuan and Zhejiang. Furthermore, principal components analysis (PCA), partial least squares discriminant analysis (PLS-DA), -test, and grey relational analysis (GRA) were applied to discriminate and evaluate the samples from Sichuan and Zhejiang based on the contents of 32 constituents. The results demonstrated that the bioactive constituents in CMD and ZMD were significantly different, and CMD performed better in the quality assessment than ZMD. This study not only provides a basic information for differentiating CMD and ZMD, but offers a new insight into comprehensive evaluation and quality control of Ophiopogonis Radix from two different producing areas.
麦冬，又称麦门冬，是一种常用的中药和功能性保健品。在中国，有两种麦冬产品主要产于四川和浙江，分别称为“川麦冬”和“浙麦冬”。为了区分和评价川麦冬和浙麦冬的质量，建立了一种基于超快速液相色谱-三重四极杆线性离子阱质谱联用（UFLC-QTRAP-MS/MS）的分析方法，用于同时测定 27 个来自四川和浙江的 27 个麦冬样品中的 32 种成分，包括 4 种甾体皂苷、3 种异黄酮、15 种氨基酸和 10 种核苷。此外，基于 32 种成分的含量，采用主成分分析（PCA）、偏最小二乘判别分析（PLS-DA）、t 检验和灰色关联分析（GRA）对来自四川和浙江的样品进行区分和评价。结果表明，川麦冬和浙麦冬中的生物活性成分存在显著差异，且在质量评价方面，川麦冬优于浙麦冬。本研究不仅为区分川麦冬和浙麦冬提供了基本信息，也为全面评价和质量控制来自两个不同产地的麦冬提供了新的思路。
