• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

羟基--山椒素通过调节 PI3K/Akt 信号通路抑制氧化应激诱导的凋亡对 HO-刺激的 PC12 细胞具有保护作用。

Hydroxy--sanshool Possesses Protective Potentials on HO-Stimulated PC12 Cells by Suppression of Oxidative Stress-Induced Apoptosis through Regulation of PI3K/Akt Signal Pathway.

机构信息

School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611130, China.

出版信息

Oxid Med Cell Longev. 2020 Jul 9;2020:3481758. doi: 10.1155/2020/3481758. eCollection 2020.

DOI:10.1155/2020/3481758
PMID:32695254
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7368233/
Abstract

pericarp is a commonly used herbal medicine in China with effects of anti-inflammatory and analgesic, improving learning and memory ability, while hydroxy--sanshool (HAS) is the most important active ingredient of pericarps. The purpose of this study was to investigate the neuroprotective effect of HAS and its related possible mechanisms using a HO-stimulated PC12 cell model. CCK-8 assay results showed that HAS had a significant protective effect on HO-stimulated PC12 cells without obvious cytotoxicity on normal PC12 cells. Flow cytometry and fluorescence microscope (DAPI staining and DCFH-DA staining) indicated that HAS could reduce the HO-induced apoptosis in PC12 cells via reduction of intracellular ROS and increase of mitochondrial membrane potential (MMP). Subsequently, results of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) determination suggested that HAS could increase the enzyme activities of SOD, CAT, and GSH-Px whereas it could decrease the MDA contents in HO-stimulated PC12 cells. Furthermore, the western blotting assays showed that HAS could upregulate the expressions of p-PI3k, Akt, p-Akt, and Bcl-2, while it could downregulate the expressions of cleaved caspase-3 and Bax in HO-stimulated PC12 cells. Collectively, it could be concluded according to our results that HAS possesses protective potentials on HO-stimulated PC12 cells through suppression of oxidative stress-induced apoptosis via regulation of PI3K/Akt signal pathway.

摘要

果皮是中国常用的草药，具有抗炎、镇痛、改善学习和记忆能力的作用，而羟基-山椒素（HAS）是果皮中最重要的活性成分。本研究旨在通过 HO 刺激的 PC12 细胞模型探讨 HAS 的神经保护作用及其相关可能机制。CCK-8 测定结果表明，HAS 对 HO 刺激的 PC12 细胞具有显著的保护作用，对正常 PC12 细胞无明显细胞毒性。流式细胞术和荧光显微镜（DAPI 染色和 DCFH-DA 染色）表明，HAS 可通过降低细胞内 ROS 和增加线粒体膜电位（MMP）来减少 HO 诱导的 PC12 细胞凋亡。随后，丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）测定结果表明，HAS 可增加 SOD、CAT 和 GSH-Px 的酶活性，同时降低 HO 刺激的 PC12 细胞中的 MDA 含量。此外，Western blot 检测结果表明，HAS 可上调 p-PI3k、Akt、p-Akt 和 Bcl-2 的表达，同时下调 HO 刺激的 PC12 细胞中 cleaved caspase-3 和 Bax 的表达。综上所述，根据我们的结果可以得出结论，HAS 通过调节 PI3K/Akt 信号通路抑制氧化应激诱导的细胞凋亡，对 HO 刺激的 PC12 细胞具有保护作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547b/7368233/78ba3e763059/OMCL2020-3481758.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547b/7368233/3ec4e7d635d9/OMCL2020-3481758.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547b/7368233/1642efa61a07/OMCL2020-3481758.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547b/7368233/a574fd3dcf41/OMCL2020-3481758.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547b/7368233/e18c1df81dcd/OMCL2020-3481758.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547b/7368233/4bc37d30f563/OMCL2020-3481758.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547b/7368233/222ce566ca0b/OMCL2020-3481758.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547b/7368233/601842226a0b/OMCL2020-3481758.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547b/7368233/78ba3e763059/OMCL2020-3481758.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547b/7368233/3ec4e7d635d9/OMCL2020-3481758.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547b/7368233/1642efa61a07/OMCL2020-3481758.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547b/7368233/a574fd3dcf41/OMCL2020-3481758.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547b/7368233/e18c1df81dcd/OMCL2020-3481758.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547b/7368233/4bc37d30f563/OMCL2020-3481758.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547b/7368233/222ce566ca0b/OMCL2020-3481758.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547b/7368233/601842226a0b/OMCL2020-3481758.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/547b/7368233/78ba3e763059/OMCL2020-3481758.008.jpg

相似文献

1
Hydroxy--sanshool Possesses Protective Potentials on HO-Stimulated PC12 Cells by Suppression of Oxidative Stress-Induced Apoptosis through Regulation of PI3K/Akt Signal Pathway.羟基--山椒素通过调节 PI3K/Akt 信号通路抑制氧化应激诱导的凋亡对 HO-刺激的 PC12 细胞具有保护作用。
Oxid Med Cell Longev. 2020 Jul 9;2020:3481758. doi: 10.1155/2020/3481758. eCollection 2020.
2
Antiepileptic Effects of Protein-Rich Extract from on Mice and Its Protective Effects against HO-Induced Oxidative Damage in PC12 Cells via Regulating PI3K/Akt Signaling Pathways.天麻蛋白提取物对小鼠的抗癫痫作用及其通过调节 PI3K/Akt 信号通路对 HO 诱导的 PC12 细胞氧化损伤的保护作用。
Oxid Med Cell Longev. 2019 May 6;2019:7897584. doi: 10.1155/2019/7897584. eCollection 2019.
3
The Protective Effect of Aspirin Eugenol Ester on Oxidative Stress to PC12 Cells Stimulated with HO through Regulating PI3K/Akt Signal Pathway.阿司匹林丁香酚酯通过调控 PI3K/Akt 信号通路对 HO 刺激的 PC12 细胞氧化应激的保护作用。
Oxid Med Cell Longev. 2021 Jun 24;2021:5527475. doi: 10.1155/2021/5527475. eCollection 2021.
4
Protection afforded by quercetin against H2O2-induced apoptosis on PC12 cells via activating PI3K/Akt signal pathway.槲皮素通过激活PI3K/Akt信号通路对H2O2诱导的PC12细胞凋亡的保护作用。
J Recept Signal Transduct Res. 2016;36(1):98-102. doi: 10.3109/10799893.2015.1049363. Epub 2015 Sep 28.
5
Protective effects of hydroxy-α-sanshool from the pericarp of Zanthoxylum bungeanum Maxim. On D-galactose/AlCl-induced Alzheimer's disease-like mice via Nrf2/HO-1 signaling pathways.花椒果皮中羟基-α-山椒素通过 Nrf2/HO-1 信号通路对 D-半乳糖/AlCl3 诱导的阿尔茨海默病样小鼠的保护作用。
Eur J Pharmacol. 2022 Jan 5;914:174691. doi: 10.1016/j.ejphar.2021.174691. Epub 2021 Dec 8.
6
Grape seed proanthocyanidins protect PC12 cells from hydrogen peroxide-induced damage via the PI3K/AKT signaling pathway.葡萄籽原花青素通过 PI3K/AKT 信号通路保护 PC12 细胞免受过氧化氢诱导的损伤。
Neurosci Lett. 2021 Apr 17;750:135793. doi: 10.1016/j.neulet.2021.135793. Epub 2021 Mar 2.
7
Rutin attenuates HO-induced oxidation damage and apoptosis in Leydig cells by activating PI3K/Akt signal pathways.芦丁通过激活 PI3K/Akt 信号通路减轻 HO 诱导的间质细胞瘤氧化损伤和细胞凋亡。
Biomed Pharmacother. 2017 Apr;88:500-506. doi: 10.1016/j.biopha.2017.01.066. Epub 2017 Jan 23.
8
Neuroprotective Effects of Deuterium-Depleted Water (DDW) Against HO-Induced Oxidative Stress in Differentiated PC12 Cells Through the PI3K/Akt Signaling Pathway.氘耗尽水（DDW）通过 PI3K/Akt 信号通路对 HO 诱导的分化 PC12 细胞氧化应激的神经保护作用。
Neurochem Res. 2020 May;45(5):1034-1044. doi: 10.1007/s11064-020-02978-4. Epub 2020 Feb 3.
9
Antiepileptic Effects of Cicadae Periostracum on Mice and Its Antiapoptotic Effects in HO-Stimulated PC12 Cells via Regulation of PI3K/Akt/Nrf2 Signaling Pathways.蝉蜕对小鼠的抗癫痫作用及其通过调节 PI3K/Akt/Nrf2 信号通路对 HO 刺激 PC12 细胞的抗凋亡作用。
Oxid Med Cell Longev. 2021 Jul 20;2021:5598818. doi: 10.1155/2021/5598818. eCollection 2021.
10
Neuroprotective effects of macranthoin G from Eucommia ulmoides against hydrogen peroxide-induced apoptosis in PC12 cells via inhibiting NF-κB activation.杜仲中杜仲胶G通过抑制NF-κB激活对过氧化氢诱导的PC12细胞凋亡的神经保护作用。
Chem Biol Interact. 2014 Dec 5;224:108-16. doi: 10.1016/j.cbi.2014.10.011. Epub 2014 Oct 19.

引用本文的文献

1
Bta-Let-7d Modulation of Oxidative Stress Induced by Potassium Permanganate in Bovine Endometrial Cells via IGF1R/PI3K/AKT Signaling Pathway.Bta-Let-7d通过IGF1R/PI3K/AKT信号通路对牛子宫内膜细胞中高锰酸钾诱导的氧化应激的调节作用
Antioxidants (Basel). 2025 Apr 8;14(4):444. doi: 10.3390/antiox14040444.
2
Integrated metabolomics and transcriptomics reveal the potential of hydroxy-alpha-sanshool in alleviating insulin resistance.整合代谢组学和转录组学揭示了羟基-α-山嵛菜碱在缓解胰岛素抵抗方面的潜力。
Mol Med. 2025 Feb 21;31(1):76. doi: 10.1186/s10020-025-01129-1.
3
Ursonic Acid Ameliorates HO-Induced Oxidative Damage in PC12 Cells and Prolonged the Lifespan in C. elegans by Activating MAPKs/Nrf2/HO-1 Signaling Pathway.

本文引用的文献

1
Alterations of the interactome of Bcl-2 proteins in breast cancer at the transcriptional, mutational and structural level.Bcl-2 蛋白相互作用组在乳腺癌中在转录、突变和结构水平上的改变。
PLoS Comput Biol. 2019 Dec 11;15(12):e1007485. doi: 10.1371/journal.pcbi.1007485. eCollection 2019 Dec.
2
Apoptosis Induction of Fibroblast-Like Synoviocytes Is an Important Molecular-Mechanism for Herbal Medicine along with its Active Components in Treating Rheumatoid Arthritis.成纤维样滑膜细胞凋亡诱导是治疗类风湿关节炎的中药及其有效成分的重要分子机制。
Biomolecules. 2019 Nov 28;9(12):795. doi: 10.3390/biom9120795.
3
Protective effect of Photobiomodulation Therapy and Bone Marrow Stromal Stem Cells Conditioned Media on Pheochromocytoma Cell Line 12 Against Oxidative Stress Induced by Hydrogen Peroxide.
乌索酸通过激活丝裂原活化蛋白激酶/核因子E2相关因子2/血红素加氧酶-1信号通路减轻HO诱导的PC12细胞氧化损伤并延长秀丽隐杆线虫的寿命。
Mol Neurobiol. 2025 May;62(5):6598-6611. doi: 10.1007/s12035-025-04701-2. Epub 2025 Jan 23.
4
Preconditioning of Mesenchymal Stem Cells Enhances the Neuroprotective Effects of Their Conditioned Medium in an Alzheimer's Disease In Vitro Model.间充质干细胞的预处理增强了其条件培养基在阿尔茨海默病体外模型中的神经保护作用。
Biomedicines. 2024 Oct 2;12(10):2243. doi: 10.3390/biomedicines12102243.
5
Pharmacological activities of L. plants and its exploitation and utilization.唇形科植物的药理活性及其开发与利用。
Heliyon. 2024 Jun 18;10(12):e33207. doi: 10.1016/j.heliyon.2024.e33207. eCollection 2024 Jun 30.
6
Studies on the Effects of Fermentation on the Phenolic Profile and Biological Activity of Three Cultivars of Kale.发酵对三种羽衣甘蓝品种酚类成分及生物活性影响的研究
Molecules. 2024 Apr 11;29(8):1727. doi: 10.3390/molecules29081727.
7
Protective Effects of Four Structurally Distinct Sanshools Ameliorate Dextran Sodium Sulfate-Induced Ulcerative Colitis by Restoring Intestinal Barrier Function and Modulating the Gut Microbiota.四种结构不同的花椒酰胺通过恢复肠道屏障功能和调节肠道微生物群改善葡聚糖硫酸钠诱导的溃疡性结肠炎的保护作用。
Antioxidants (Basel). 2024 Jan 25;13(2):153. doi: 10.3390/antiox13020153.
8
Research advances of polyphenols in inflammatory diseases.多酚类物质在炎症性疾病中的研究进展。
Front Immunol. 2024 Jan 26;15:1305886. doi: 10.3389/fimmu.2024.1305886. eCollection 2024.
9
WGX50 mitigates doxorubicin-induced cardiotoxicity through inhibition of mitochondrial ROS and ferroptosis.WGX50 通过抑制线粒体 ROS 和铁死亡减轻阿霉素诱导的心脏毒性。
J Transl Med. 2023 Nov 17;21(1):823. doi: 10.1186/s12967-023-04715-1.
10
Pioglitazone protects PC12 cells against oxidative stress injury: An study of its antiapoptotic effects via the PPARγ pathway.吡格列酮保护PC12细胞免受氧化应激损伤：通过PPARγ途径对其抗凋亡作用的研究。
Exp Ther Med. 2023 Sep 22;26(5):522. doi: 10.3892/etm.2023.12221. eCollection 2023 Nov.
光生物调节疗法和骨髓基质干细胞条件培养基对嗜铬细胞瘤细胞系12抵抗过氧化氢诱导的氧化应激的保护作用。
J Lasers Med Sci. 2019 Summer;10(3):163-170. doi: 10.15171/jlms.2019.26. Epub 2019 Jul 6.
4
Hydroxy-α-sanshool isolated from Zanthoxylum bungeanum attenuates learning and memory impairments in scopolamine-treated mice.从花椒中分离得到的羟基-α-山椒素可减轻东莨菪碱处理小鼠的学习记忆障碍。
Food Funct. 2019 Nov 1;10(11):7315-7324. doi: 10.1039/c9fo00045c. Epub 2019 Oct 22.
5
Hyperglycemia-Induced Oxidative Stress Abrogates Remifentanil Preconditioning-Mediated Cardioprotection in Diabetic Rats by Impairing Caveolin-3-Modulated PI3K/Akt and JAK2/STAT3 Signaling.高血糖诱导的氧化应激通过损害窖蛋白 3 调节的 PI3K/Akt 和 JAK2/STAT3 信号通路来破坏瑞芬太尼预处理介导的糖尿病大鼠的心肌保护作用。
Oxid Med Cell Longev. 2019 Sep 5;2019:9836302. doi: 10.1155/2019/9836302. eCollection 2019.
6
Antiobesity, Regulation of Lipid Metabolism, and Attenuation of Liver Oxidative Stress Effects of Hydroxy--sanshool Isolated from on High-Fat Diet-Induced Hyperlipidemic Rats.从 中分离得到的羟基-三甲氧基菔酚对高脂饮食诱导的高血脂大鼠的抗肥胖、调节脂代谢和减轻肝脏氧化应激作用。
Oxid Med Cell Longev. 2019 Aug 27;2019:5852494. doi: 10.1155/2019/5852494. eCollection 2019.
7
Antiepileptic Effects of Protein-Rich Extract from on Mice and Its Protective Effects against HO-Induced Oxidative Damage in PC12 Cells via Regulating PI3K/Akt Signaling Pathways.天麻蛋白提取物对小鼠的抗癫痫作用及其通过调节 PI3K/Akt 信号通路对 HO 诱导的 PC12 细胞氧化损伤的保护作用。
Oxid Med Cell Longev. 2019 May 6;2019:7897584. doi: 10.1155/2019/7897584. eCollection 2019.
8
Atractylenolide III Attenuates Muscle Wasting in Chronic Kidney Disease via the Oxidative Stress-Mediated PI3K/AKT/mTOR Pathway.白术内酯 III 通过氧化应激介导的 PI3K/AKT/mTOR 通路减轻慢性肾脏病中的肌肉减少症。
Oxid Med Cell Longev. 2019 Apr 18;2019:1875471. doi: 10.1155/2019/1875471. eCollection 2019.
9
Neuroprotective effect of phosphocreatine on oxidative stress and mitochondrial dysfunction induced apoptosis in vitro and in vivo: Involvement of dual PI3K/Akt and Nrf2/HO-1 pathways.磷酸肌酸对氧化应激和线粒体功能障碍诱导的细胞凋亡的神经保护作用：涉及双重 PI3K/Akt 和 Nrf2/HO-1 通路。
Free Radic Biol Med. 2018 May 20;120:228-238. doi: 10.1016/j.freeradbiomed.2018.03.014. Epub 2018 Mar 17.
10
Oxidative stress and reactive oxygen species: a review of their role in ocular disease.氧化应激与活性氧：对其在眼部疾病中作用的综述
Clin Sci (Lond). 2017 Dec 4;131(24):2865-2883. doi: 10.1042/CS20171246. Print 2017 Dec 15.