Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico.
Departamento de Bioquímica, Facultad de Química, Universidad Nacional Autónoma de México, Mexico City, México.
Methods Mol Biol. 2020;2141:519-528. doi: 10.1007/978-1-0716-0524-0_26.
Unlike for structured proteins, the study of intrinsically disordered proteins (IDPs) requires selection of ad hoc assays and strategies to characterize their dynamic structure and function. Late embryogenesis abundant (LEA) proteins are important plant IDPs closely related to water-deficit stress response. Diverse hypothetical functions have been proposed for LEA proteins, such as membrane stabilizers during cold stress, oxidative regulators acting as ion metal binding molecules, and protein protectants during dehydration and cold/freezing conditions. Here we present two detailed protocols to characterize IDPs with potential protein/enzyme protection activity under partial dehydration and freeze-thaw treatments.
与结构蛋白不同，研究无规卷曲蛋白质（IDPs）需要选择特定的测定法和策略来描述其动态结构和功能。晚期胚胎丰富蛋白（LEA）是与水分亏缺应激反应密切相关的重要植物 IDP。已经提出了 LEA 蛋白的多种假设功能，例如在冷胁迫期间作为膜稳定剂，作为离子金属结合分子的氧化调节剂，以及在脱水和冷冻/冻结条件下作为蛋白质保护剂。在这里，我们提出了两个详细的方案，用于在部分脱水和冻融处理下，对具有潜在蛋白质/酶保护活性的 IDPs 进行特征描述。
