Department of Pathology and Lab Medicine, Robert Wood Johnson Medical School, Rutgers, the State University of New Jersey, New Brunswick, NJ, USA.
OptoVibronex, LLC, Mount Bethel, PA, USA.
Skin Res Technol. 2021 Mar;27(2):227-233. doi: 10.1111/srt.12921. Epub 2020 Jul 21.
The modulus and resonant frequency of cancer cells and extracellular matrix are increased in both basal cell and squamous cell carcinomas, and in addition, the collagen stiffness is increased. The organization of the extracellular matrix surrounding cancer cells is clearly different than the extracellular matrix that is seen in normal skin.
We have used vibrational optical coherence tomography (VOCT) to measure the resonant frequency and stiffness of collagen, vascular, and reorganized fibrous extracellular matrix components. Measurements of vessels and fibrotic collagen content made on basal cell carcinomas (BCCs) are compared to similar measurements made on normal skin from different anatomical locations.
Lesions with basal cell carcinomas exhibit characteristic resonant frequencies and moduli of reorganized extracellular matrix characteristic of fibrotic tissue. Cancerous lesions are characterized by dermal collagen (100 Hz), vascular (150 Hz), and fibrotic peaks (200-230 Hz). The fibrotic peak is not found in areas of normal skin.
Based on this pilot study, it is hypothesized inflammation may promote precancerous lesion formation in the surrounding extracellular matrix. Measurement of the increases in vaculature and fibrotic content may be useful in early detection of BCCs.
基底细胞癌和鳞状细胞癌中的癌细胞和细胞外基质的模量和共振频率增加，此外，胶原硬度也增加。癌细胞周围细胞外基质的组织明显不同于正常皮肤中看到的细胞外基质。
我们使用振动光学相干断层扫描（VOCT）来测量胶原、血管和重组纤维细胞外基质成分的共振频率和硬度。基底细胞癌（BCC）上的血管和纤维性胶原含量的测量结果与来自不同解剖部位的正常皮肤的类似测量结果进行了比较。
基底细胞癌病变表现出与纤维化组织特征性的重组细胞外基质的特征共振频率和模量。癌性病变的特征是真皮胶原（100 Hz）、血管（150 Hz）和纤维性峰值（200-230 Hz）。在正常皮肤区域未发现纤维性峰值。
基于这项初步研究，假设炎症可能会促进周围细胞外基质中癌前病变的形成。血管生成和纤维性含量的增加的测量可能对基底细胞癌的早期检测有用。
