Use of vibrational optical coherence tomography to measure viscoelastic properties of muscle and tendon: A new method to follow musculoskeletal injury and pathology In vivo.

The biomechanical properties of muscles and tendons in vivo are important parameters needed to understand musculoskeletal physiology and pathology. Values of the shear moduli reported for human musculoskeletal components using elastographic techniques range from several KPa to about 100 KPa and are much lower than the tensile moduli measured in vivo which are reported to be as high as several hundred MPa at high strains. In this paper we report the results of a pilot study to measure the mechanical properties of human muscles and tendons non-invasively and non-destructively in vivo using vibrational optical coherence tomography (VOCT). VOCT is a non-invasive technique that uses audible sound and reflected infrared light to measure the resonant frequency of each tissue component. Using VOCT we report that the moduli at the biceps muscle-tendon junction are about 24-30 MPa even though moduli in other anatomic locations of muscle and tendons can vary by as much as 10 MPa. It is concluded that the modulus and stress exerted by tendons and muscles at the muscle-tendon junction are similar and that deposition of fibrous tissue at the junction will lead to reduced values of the modulus leading to tissue pathology and muscle injury.