Cornea Service, Wills Eye Hospital, Sidney Kimmel Medical College at Thomas Jefferson University, Philadelphia, PA, USA.
Vickie and Jack Farber Vision Research Center, Wills Eye Hospital, Philadelphia, PA, USA.
Transl Vis Sci Technol. 2022 Jul 8;11(7):11. doi: 10.1167/tvst.11.7.11.
To determine the in vivo elastic modulus of the human cornea using vibrational optical coherence tomography (VOCT).
Vibrational analysis coupled with optical coherence tomography (OCT) was used to obtain the resonant frequency (RF) and elastic modulus of corneal structural components. VOCT corneal thickness values were measured using OCT images and correlated with corneal thickness determined with Pentacam (Oculus, Wetzlar, Germany). Moduli were obtained at two locations: central cornea (CC) and inferior cornea (IC). Measurements were obtained with and without anesthetic eye drops to assess their effect on the modulus measurements.
VOCT thickness values correlated positively (R2 = 0.97) and linearly (y = 1.039x-16.89) with those of Pentacam. Five RF peaks (1-5) were present, although their presence was variable across eyes. The RF for peaks 1 to 5 in the CC and IC ranged from 73.5 ± 4.9 to 239 ± 3 Hz and 72.1 ± 6.3 to 238 ± 4 Hz, respectively. CC and IC moduli for peaks 1 to 5 ranged from 1.023 ± 0.104 to 6.87 ± 0.33 MPa and 0.98 ± 0.15 to 6.52 ± 0.79 MPa, respectively. Topical anesthesia did not significantly alter the modulus (P > 0.05 for all), except for peak 2 in the CC (P < 0.05).
This pilot study demonstrates the utility of VOCT as an in vivo, noninvasive technology to measure the elastic modulus in human corneas. The structural origin of these moduli is hypothesized based on previous reports, and further analyses are necessary for confirmation.
This work presents VOCT as a novel approach to assess the in vivo elastic modulus of the cornea, an indicator of corneal structural integrity and health.
利用振动光相干断层扫描（VOCT）测定人眼角膜的体内弹性模量。
振动分析与光相干断层扫描（OCT）相结合，获得角膜结构成分的共振频率（RF）和弹性模量。使用 OCT 图像测量 VOCT 角膜厚度值，并与德国 Oculus 公司的 Pentacam （威茨拉尔）确定的角膜厚度进行相关。在中央角膜（CC）和下角膜（IC）两个位置进行测量。测量时使用和不使用局部麻醉滴眼液，以评估其对模量测量的影响。
VOCT 厚度值与 Pentacam 的值呈正相关（R2=0.97）和线性相关（y=1.039x-16.89）。尽管存在个体差异，但在所有眼中都存在 5 个 RF 峰（1-5）。CC 和 IC 中的 RF1 至 5 峰范围分别为 73.5±4.9 至 239±3Hz 和 72.1±6.3 至 238±4Hz。CC 和 IC 的 RF1 至 5 峰的模量范围分别为 1.023±0.104 至 6.87±0.33MPa 和 0.98±0.15 至 6.52±0.79MPa。局部麻醉滴眼剂并未显著改变模量（所有 P 值>0.05），除了 CC 的 RF2 峰（P<0.05）。
本初步研究证明了 VOCT 作为一种非侵入性活体测量人眼角膜弹性模量的技术的实用性。这些模量的结构来源基于以前的报告提出假设，需要进一步分析进行确认。
胡彧
