Ryzhmanova Yana, Oshurkova Victoria, Troshina Olga, Abashina Tatyana, Ariskina Elena, Avtukh Alexander, Shcherbakova Viktoria
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, prospect Nauki 5, Pushchino, Moscow region, 142290, Russia.
Int J Syst Evol Microbiol. 2017 Nov;67(11):4704-4709. doi: 10.1099/ijsem.0.002365. Epub 2017 Oct 6.
An anaerobic alkaliphilic, proteolytic bacterium, strain Su22, was isolated from the bottom sediment of the alkaline low mineralization lake Sulphatnoe (Selenginsky district, Buryatia, Russia). A comparative analysis of the 16S rRNA gene sequence revealed that this bacterium was closely related to Anoxynatronum sibiricum Z-7981 with a similarity of 98.1 %. Strain Su22 differed from A. sibiricum Z-7981 in its inability to use carbohydrates, peptone and amino acids as carbon sources. Strain Su22 grew over a temperature range of 20-40 °C with an optimum at 30 °C and within the pH range 7.4-11.0 with an optimum at pH 9.6. Sodium cations stimulated the growth of the strain considerably with an optimal concentration at 0.76-1.09 M. The whole-cell fatty acid profile included C16 : 1ω7c, C16 : 0 and C16 : 0 ALDE. The G+C content was 46.1 mol%. Based on the DNA-DNA hybridization level (53.2 %) and phenotypical differences between strains Su22 and Z-7981, the new isolate is thus considered to represent a novel species, for which the name Anoxynatronumburyatiense sp. nov. is proposed. The type strain is Su22 (=VKM B-2510=CECT 8731).
从碱性低矿化度的硫酸盐湖（俄罗斯布里亚特共和国色楞格区）底部沉积物中分离出一株厌氧嗜碱、蛋白水解细菌，菌株Su22。对16S rRNA基因序列的比较分析表明，该细菌与西伯利亚嗜钠厌氧杆菌Z-7981密切相关，相似度为98.1%。菌株Su22与西伯利亚嗜钠厌氧杆菌Z-7981的不同之处在于它不能利用碳水化合物、蛋白胨和氨基酸作为碳源。菌株Su22在20-40°C的温度范围内生长，最适温度为30°C，在pH值7.4-11.0的范围内生长，最适pH值为9.6。钠离子对该菌株的生长有显著促进作用，最佳浓度为0.76-1.09 M。全细胞脂肪酸谱包括C16:1ω7c、C16:0和C16:0醛。G+C含量为46.1 mol%。基于DNA-DNA杂交水平（53.2%）以及菌株Su22和Z-7981之间的表型差异，因此认为该新分离株代表一个新物种，为此提出新名称伯利亚嗜钠厌氧杆菌（Anoxynatronum buryatiense）sp. nov.。模式菌株为Su22（=VKM B-2510=CECT 8731）。
