Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.
Department of Central Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.
Biol Reprod. 2020 Oct 29;103(5):918-926. doi: 10.1093/biolre/ioaa124.
An ideal research model plays a vital role in studying the pathogenesis of a disease. At present, the most widely used endometrial disease models are cell lines and animal models. As a novel studying model, organoids have already been applied for the study of various diseases, such as disorders related to the liver, small intestine, colon, and pancreas, and have been extended to the endometrium. After a long period of exploration by predecessors, endometrial organoids (EOs) technology has gradually matured and maintained genetic and phenotypic stability after long-term expansion. Compared with cell lines and animal models, EOs have high stability and patient specificity. These not only effectively and veritably reflects the pathophysiology of a disease, but also can be used in preclinical drug screening, combined with patient derived xenografts (PDXs). Indeed, there are still many limitations for EOs. For example, the co-culture system of EOs with stromal cells, immune cell, or vascular cells is not mature, and endometrial cancer organoids have a lower success rate, which should be improved in the future. The investigators predict that EOs will play a significant role in the study of endometrium-related diseases.
理想的研究模型在研究疾病发病机制方面起着至关重要的作用。目前，最广泛使用的子宫内膜疾病模型是细胞系和动物模型。作为一种新型研究模型，类器官已被应用于各种疾病的研究，如与肝脏、小肠、结肠和胰腺相关的疾病，并已扩展到子宫内膜。经过前人的长期探索，子宫内膜类器官（EOs）技术逐渐成熟，经过长期扩增后保持遗传和表型稳定性。与细胞系和动物模型相比，EOs 具有更高的稳定性和患者特异性。这不仅有效地、真实地反映了疾病的病理生理学，而且可以用于临床前药物筛选，并与患者来源的异种移植物（PDXs）结合使用。事实上，EOs 仍然存在许多局限性。例如，EOs 与基质细胞、免疫细胞或血管细胞的共培养系统尚不成熟，子宫内膜癌类器官的成功率较低，这在未来需要加以改进。研究人员预测，EOs 将在子宫内膜相关疾病的研究中发挥重要作用。
