Graduate Institute of Biomedical Science, China Medical University, Taichung 40402, Taiwan, R.O.C.
Translational Medicine Research, China Medical University Hospital, Taichung 40402, Taiwan, R.O.C.
ACS Appl Mater Interfaces. 2020 Oct 7;12(40):44393-44406. doi: 10.1021/acsami.0c05747. Epub 2020 Sep 25.
Controlling the behavior of mesenchymal stem cells (MSCs) through topographic patterns is an effective approach for stem cell studies. We, herein, reported a facile method to create a dopamine (DA) pattern on poly(dimethylsiloxane) (PDMS). The topography of micropatterned DA was produced on PDMS after plasma treatment. The grid-topographic-patterned surface of PDMS-DA (PDMS-DA-P) was measured for adhesion force and Young's modulus by atomic force microscopy. The surface of PDMS-DA-P demonstrated less stiff and more elastic characteristics compared to either nonpatterned PDMS-DA or PDMS. The PDMS-DA-P evidently enhanced the differentiation of MSCs into various tissue cells, including nerve, vessel, bone, and fat. We further designed comprehensive experiments to investigate adhesion, proliferation, and differentiation of MSCs in response to PDMS-DA-P and showed that the DA-patterned surface had good biocompatibility and did not activate macrophages or platelets in vitro and had low foreign body reaction in vivo. Besides, it protected MSCs from apoptosis as well as excessive reactive oxygen species (ROS) generation. Particularly, the patterned surface enhanced the differentiation capacity of MSCs toward neural and endothelial cells. The stromal cell-derived factor-1α/CXantiCR4 pathway may be involved in mediating the self-recruitment and promoting the differentiation of MSCs. These findings support the potential application of PDMS-DA-P in either cell treatment or tissue repair.
通过形貌图案控制间充质干细胞（MSCs）的行为是干细胞研究的有效方法。我们在此报告了一种在聚二甲基硅氧烷（PDMS）上制造多巴胺（DA）图案的简便方法。等离子体处理后，在 PDMS 上产生了具有微图案的 DA 形貌。通过原子力显微镜测量 PDMS-DA（PDMS-DA-P）的网格形貌表面的粘附力和杨氏模量。与非图案化 PDMS-DA 或 PDMS 相比，PDMS-DA-P 的表面表现出较小的刚性和更大的弹性特性。PDMS-DA-P 明显增强了 MSCs 向各种组织细胞（包括神经、血管、骨骼和脂肪）的分化。我们进一步设计了综合实验来研究 MSCs 对 PDMS-DA-P 的粘附、增殖和分化，结果表明，DA 图案化表面具有良好的生物相容性，在体外不会激活巨噬细胞或血小板，在体内也不会引起异物反应。此外，它还可以保护 MSCs 免受细胞凋亡和过多活性氧（ROS）的产生。特别是，图案化表面增强了 MSCs 向神经和内皮细胞的分化能力。基质细胞衍生因子-1α/CXantiCR4 途径可能参与介导细胞的自我募集和促进 MSCs 的分化。这些发现支持 PDMS-DA-P 在细胞治疗或组织修复中的潜在应用。
