Joseph Georghios, Orme Rowan P, Kyriacou Theocharis, Fricker Rosemary A, Roach Paul
Institute for Science and Technology in Medicine, and School of Medicine, Keele University, Keele, Staffs ST5 5BG, U.K.
School of Computing and Mathematics, Keele University, Keele, Staffs ST5 5BG, U.K.
ACS Omega. 2021 Jul 19;6(30):19901-19910. doi: 10.1021/acsomega.1c02796. eCollection 2021 Aug 3.
The characteristics of a material's surface are extremely important when considering their interactions with biological species. Despite surface chemistry playing a critical role in mediating the responses of cells, there remains no single rule which dictates absolute performance; this is particularly challenging when considering the response of differing cell types to a range of materials. Here, we highlight the functional behavior of neural stem cells presented as neurospheres, with respect to a range of alkane-based self-assembled monolayers presenting different functional groups: OH, COH, NH, phenyl, CH, SH, and laminin. The influence of chemical cues was examined in terms of neurosphere spreading on each of these defined surfaces (cell adhesion and migration capacity) and neuronal versus glial marker expression. Measurements were made over a time series of 3, 5, and 7 days, showing a dynamic nature to the initial responses observed after seeding. While OH surfaces presented an excellent platform for glial migration, larger proportions of cells expressing neuronal β-tubulin were found on SH- and laminin-coated surfaces. Axonal elongation was found to be initially similar on all surfaces with neurite lengths having a wider spread predominantly on NH- and laminin-presenting surfaces. A generalized trend could not be found to correlate cellular responses with surface wettability, lipophilicity (log ), or charge/ionizability (p ). These results highlight the potential for chemical cues to direct primary neural stem cell responses in contact with the defined materials. New biomaterials which control specific cell culture characteristics will streamline the up-scale manufacture of cellular therapies, with the enrichment of the required populations resulting from a defined material interaction.
在考虑材料与生物物种的相互作用时，材料表面的特性极其重要。尽管表面化学在介导细胞反应中起着关键作用，但尚无单一规则能决定绝对性能；在考虑不同细胞类型对一系列材料的反应时，这尤其具有挑战性。在这里，我们重点介绍了呈神经球形式的神经干细胞相对于一系列具有不同官能团的基于烷烃的自组装单分子层的功能行为：OH、COH、NH、苯基、CH、SH和层粘连蛋白。从神经球在这些特定表面上的铺展（细胞粘附和迁移能力）以及神经元与神经胶质标记物表达方面研究了化学信号的影响。在3天、5天和7天的时间序列上进行了测量，显示出接种后观察到的初始反应具有动态性质。虽然OH表面为神经胶质细胞迁移提供了一个极好的平台，但在SH和层粘连蛋白包被的表面上发现表达神经元β-微管蛋白的细胞比例更高。发现轴突伸长在所有表面上最初相似，神经突长度在主要呈NH和层粘连蛋白的表面上分布更广泛。无法找到将细胞反应与表面润湿性、亲脂性（log ）或电荷/离子化能力（p ）相关联的普遍趋势。这些结果突出了化学信号在引导原代神经干细胞与特定材料接触时的反应方面的潜力。控制特定细胞培养特性的新型生物材料将简化细胞疗法的大规模生产，通过特定材料相互作用实现所需细胞群体的富集。
