Takeda Keita, Nagai Hideaki, Suzukawa Maho, Sekiguchi Ryo, Akashi Shunsuke, Sato Ryota, Narumoto Osamu, Kawashima Masahiro, Suzuki Junko, Ohshima Nobuharu, Yamane Akira, Tamura Atsuhisa, Matsui Hirotoshi, Tohma Shigeto
Center for Pulmonary Diseases, National Hospital Organization Tokyo National Hospital; Department of Basic Mycobacteriology, Graduate School of Biomedical Science, Nagasaki University.
Center for Pulmonary Diseases, National Hospital Organization Tokyo National Hospital.
J Infect Chemother. 2020 Nov;26(11):1205-1212. doi: 10.1016/j.jiac.2020.06.019. Epub 2020 Jul 19.
This study evaluated the efficacy of the following interferon (IFN)-γ release assays (IGRAs): QuantiFERON-TB Gold Plus (QFT-Plus), QFT-Gold In-Tube (QFT-GIT), and T-SPOT. TB (T-SPOT) with the quantitative values of IFN-γ response.
Blood samples were collected from patients with active tuberculosis (TB), latent TB infection (LTBI), individuals with previous TB infection, and healthy volunteers enrolled between May 2017 and June 2018.
IGRAs results were analyzed in 175 subjects (76 had active TB, 14 had LTBI, 35 had prior TB infection, and 50 were healthy). QFT-Plus and QFT-GIT revealed equal efficacy for IFN-γ values, and the IFN-γ response in QFTs tended to increase with the spot counts in T-SPOT, with similar high sensitivities (approximately 90%) in the active TB group. The test concordance of two of three IGRAs was optimal among all subjects (κ coefficients: 0.82-0.96). Additionally, the median quantitative values of IFN-γ with QFT-Plus and QFT-GIT were higher in the active TB group than in the LTBI and previous TB groups.
Three IGRAs showed equivalent efficacy with high sensitivities and higher IFN-γ response in active TB group than that in non-active TB group.
本研究评估了以下干扰素（IFN）-γ释放检测（IGRA）的疗效：结核感染T细胞检测升级版（QFT-Plus）、结核感染T细胞检测管内检测法（QFT-GIT）和结核感染T细胞检测（T-SPOT），并分析了IFN-γ反应的定量值。
收集2017年5月至2018年6月期间纳入的活动性结核病（TB）患者、潜伏性结核感染（LTBI）患者、既往有结核感染的个体和健康志愿者的血样。
对175名受试者（76例活动性TB、14例LTBI、35例既往有结核感染、50例健康）的IGRA结果进行了分析。QFT-Plus和QFT-GIT在IFN-γ值方面显示出相同的疗效，QFT中的IFN-γ反应倾向于随着T-SPOT中的斑点数增加，在活动性TB组中具有相似的高敏感性（约90%）。在所有受试者中，三种IGRA中的两种检测一致性最佳（κ系数：0.82-0.96）。此外，活动性TB组中QFT-Plus和QFT-GIT的IFN-γ定量值中位数高于LTBI组和既往有结核感染组。
三种IGRA显示出等效疗效，具有高敏感性，且活动性TB组的IFN-γ反应高于非活动性TB组。
