School of Life Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom.
Institute of Molecular Botany, Ulm University, 89069 Ulm, Germany.
Plant Cell. 2020 Sep;32(9):2742-2762. doi: 10.1105/tpc.20.00154. Epub 2020 Jul 22.
While root diseases are among the most devastating stresses in global crop production, our understanding of root immunity is still limited relative to our knowledge of immune responses in leaves. Considering that root performance is based on the concerted functions of its different cell types, we undertook a cell type-specific transcriptome analysis to identify gene networks activated in epidermis, cortex, and pericycle cells of Arabidopsis () roots challenged with two immunity elicitors, the bacterial flagellin-derived flg22 and the endogenous Pep1 peptide. Our analyses revealed distinct immunity gene networks in each cell type. To further substantiate our understanding of regulatory patterns underlying these cell type-specific immunity networks, we developed a tool to analyze paired transcription factor binding motifs in the promoters of cell type-specific genes. Our study points toward a connection between cell identity and cell type-specific immunity networks that might guide cell types in launching immune response according to the functional capabilities of each cell type.
虽然根部病害是全球作物生产中最具破坏性的胁迫因素之一，但相对于我们对叶片免疫反应的了解，我们对根部免疫的理解仍然有限。考虑到根系的性能基于其不同细胞类型的协同功能，我们进行了细胞类型特异性转录组分析，以鉴定在拟南芥根表皮、皮层和周皮细胞中被两种免疫激发子（细菌鞭毛衍生的 flg22 和内源性 Pep1 肽）挑战时激活的基因网络。我们的分析揭示了每种细胞类型中独特的免疫基因网络。为了进一步证实我们对这些细胞类型特异性免疫网络背后的调控模式的理解，我们开发了一种工具来分析细胞类型特异性基因启动子中配对转录因子结合基序。我们的研究表明，细胞身份和细胞类型特异性免疫网络之间存在联系，这可能指导细胞类型根据每个细胞类型的功能能力启动免疫反应。
