Key Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.
Institute of Agri-resources and Regional Planning, CAAS, Beijing 100081, PR China.
Int J Syst Evol Microbiol. 2020 Aug;70(8):4808-4815. doi: 10.1099/ijsem.0.004350. Epub 2020 Jul 23.
A Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacterial strain, O9, was isolated from a forest soil sample collected at Dai, Xishuangbanna, Yunnan Province, PR China. Strain O9 grew optimally at pH 7.0, at 28‒30 °C and in the absence of NaCl. 16S rRNA gene sequence analysis placed strain O9 within the genus of the family with KP01 (97.8 %), JN53 (97.7 %), JS16-4 (97.4 %), JN246 (97.3 %) and DHOC24 (97.3 %) as its closest relatives. Strain O9 hydrolysed casein, gelatin and Tween 80. It could not assimilate l-arabinose, l-rhamnose, sucrose, melibiose, gentiobiose or d-fructose as a carbon source. It was negative for esterase lipase (C8) and -glucosidase. Phosphatidylethanolamine was the predominant polar lipid. The major respiratory quinone of strain O9 was MK-7. Its major fatty acids were iso-C (34.2 %), C 5 (20.9 %) and iso-C 3-OH (12.6 %). The genomic DNA G+C content of strain O9 was 49.0 mol% based on total genome calculations. The average nucleotide identity score between the genomic sequence of strain O9 and that of KP01 was 72.9%. The Genome-to-Genome Distance Calculator showed that DNA‒DNA hybridization values for strain O9 and KP01 were 13.6, 21.1 and 14.4%, respectively. Based on the polyphasic taxonomic data, strain O9 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is O9 (=CGMCC 1.12462=KCTC 32404).
一株革兰氏阴性、严格需氧、不运动的杆状细菌菌株 O9，从中国云南省西双版纳傣族自治州大渡采集的森林土壤样本中分离得到。菌株 O9 在 pH 值为 7.0、28-30°C 和无 NaCl 的条件下生长最佳。16S rRNA 基因序列分析将菌株 O9 置于 科，与 KP01（97.8%）、JN53（97.7%）、JS16-4（97.4%）、JN246（97.3%）和 DHOC24（97.3%）亲缘关系最近。菌株 O9 可水解酪蛋白、明胶和吐温 80。它不能同化阿拉伯糖、鼠李糖、蔗糖、棉子糖、龙胆二糖或 D-果糖作为碳源。它对酯酶脂肪酶（C8）和-β-葡萄糖苷酶呈阴性。磷脂酰乙醇胺是主要的极性脂。菌株 O9 的主要呼吸醌是 MK-7。其主要脂肪酸为 iso-C（34.2%）、C 5（20.9%）和 iso-C 3-OH（12.6%）。根据全基因组计算，菌株 O9 的基因组 DNA G+C 含量为 49.0 mol%。菌株 O9 基因组序列与 KP01 基因组序列的平均核苷酸同一性得分为 72.9%。基因组到基因组距离计算器显示，菌株 O9 和 KP01 之间的 DNA-DNA 杂交值分别为 13.6%、21.1%和 14.4%。基于多相分类学数据，菌株 O9 代表了 属的一个新种，因此建议将其命名为 sp. nov.。模式菌株为 O9（=CGMCC 1.12462=KCTC 32404）。
