Yao Yuxin, Zhong Xinhua, Li Huixian, Fan Weilin, Xiang Qi, Huang Yadong
College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China.
Institute of Biomedicine and Guangdong Provincial Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou 510632, PR China.
Int J Syst Evol Microbiol. 2021 Jul;71(7). doi: 10.1099/ijsem.0.004865.
A novel bacterial strain, designated K2CV101002-2, was isolated from forest soil collected at Dinghushan Biosphere Reserve, Guangdong Province, PR China. Phylogenetic analyses based on 16S rRNA gene sequences showed that it belonged to the genus and was most closely related to KP01 (99.0 %), followed by Mgbs1 (98.3 %) and O9 (98.1 %). The draft genome sequence was 6.8 Mb long with a relative low G+C content of 39.8 mol%. The average nucleotide identity and digital DNA-DNA hybridization values between the novel strain and closely related type strains were 71.4‒76.2 % and 18.4‒19.6 %, respectively. Meanwhile the corresponding values between Mgbs1 and O9 were 98.6 and 88.1 %, respectively. The novel strain contained iso-C, C 5 and iso-C 3-OH as the major fatty acids and MK-7 as the predominant respiratory quinone. The polyphasic study clearly supported that strain K2CV101002-2 represents a new species of the genus , for which the name sp. nov. (type strain K2CV101002-2=GDMCC 1.1288=JCM 32696) is proposed. In addition, Goh . 2020 should be taken as a later heterotypic synonym of Ping . 2020.
从中国广东省鼎湖山生物圈保护区采集的森林土壤中分离出一株新型细菌菌株，命名为K2CV101002-2。基于16S rRNA基因序列的系统发育分析表明，它属于该属，与KP01关系最为密切（相似度为99.0%），其次是Mgbs1（98.3%）和O9（98.1%）。基因组草图序列长6.8 Mb，G+C含量相对较低，为39.8 mol%。该新型菌株与密切相关的模式菌株之间的平均核苷酸同一性和数字DNA-DNA杂交值分别为71.4‒76.2%和18.4‒19.6%。同时，Mgbs1和O9之间的相应值分别为98.6%和88.1%。该新型菌株以异-C、C5和异-C3-OH作为主要脂肪酸，以MK-7作为主要呼吸醌。多相研究明确支持菌株K2CV101002-2代表该属的一个新物种，为此提出新物种名称sp. nov.（模式菌株K2CV101002-2 = GDMCC 1.1288 = JCM 32696）。此外，Goh. 2020应被视为Ping. 2020较晚的异名。
