Science and Math Department, Judson University, Elgin, 60123, Illinois, USA.
Chemistry and Biochemistry Department, Albright College, Reading, 19604, Pennsylvania, USA.
J Eukaryot Microbiol. 2020 Nov;67(6):687-690. doi: 10.1111/jeu.12819. Epub 2020 Aug 13.
We describe an inexpensive magnetic cell patterning method as a tool for protozoologists. The ciliate Vorticella convallaria is useful for various biofluidics applications. Here, we show that V. convallaria will ingest metal beads and that permanent magnets can be used to pattern cells in Petri dishes or a microfluidic device. Patterning is reversibly achieved by placing magnets at the point of desired cell attachment. Analogous magnetic manipulation could be performed using other phagocytic cells.
我们描述了一种廉价的磁性细胞图案化方法，作为原生动物学家的工具。喇叭虫 Vorticella convallaria 可用于各种生物流体应用。在这里，我们表明 V. convallaria 可以摄取金属珠，并且可以使用永久磁铁在培养皿或微流控装置中对细胞进行图案化。通过将磁铁放置在所需细胞附着的位置，可以可逆地实现图案化。类似的磁操作可以使用其他吞噬细胞来进行。
