State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, China; National Engineering Laboratory for Industrial Wastewater Treatment, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, China.
State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, China; National Engineering Laboratory for Industrial Wastewater Treatment, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai, China.
Sci Total Environ. 2020 Nov 20;744:140785. doi: 10.1016/j.scitotenv.2020.140785. Epub 2020 Jul 7.
Simple cryopreservation of anaerobic ammonium-oxidation (anammox) consortia has become a promising preservation technology for the fast start-up of the anammox process. Here, we use genome-resolved metagenomics and metatranscriptomics to understand of the microbial interaction in a simple and effective resuscitation process for long-term cryopreserved anammox consortia by sequential addition of anammox SBR supernatant. Performance results showed that sequential addition of anammox supernatant significantly reduced the resuscitation time of the granule-based anammox process from 40 to 20 days. Genome-centric metagenomics were used to recover 19 high-quality draft genomes of anammox and heterotrophic bacteria. Comparative metatranscriptomic analysis was conducted to examine the gene expression of Candidatus Kuenenia stuttgartiensis, the dominant anammox bacterium, and heterotrophic bacteria to better understand their potential interactions. Proteobacteria-affiliated bacteria found in the supernatant were highly active in producing the secondary metabolites molybdopterin cofactor and folate which are needed for growth of the auxotrophic anammox bacteria. In addition, the significantly higher expression levels of hzsA and CO-fixtion genes in the Candidatus Kuenenia genome indicated the anammox bacteria were likely more active and growing faster after sequential anammox supernatant addition during the resuscitation process. The resuscitation treatment pulse assays confirmed that sequential addition of supernatant was an effective way for the rapid resuscitation of anammox consortia. Our findings offer the first evidence of cross-feeding during the rapid resuscitation of cryopreserved anammox consortia.
简单的冷冻保存厌氧氨氧化（anammox）菌已成为 anammox 工艺快速启动的一种有前途的保存技术。在这里，我们使用基因组解析宏基因组学和宏转录组学来了解在通过连续添加厌氧氨氧化 SBR 上清液对长期冷冻保存的 anammox 菌进行简单有效的复苏过程中的微生物相互作用。性能结果表明，连续添加 anammox 上清液可将基于颗粒的 anammox 工艺的复苏时间从 40 天缩短至 20 天。基于基因组的宏基因组学用于回收 19 株高质量的 anammox 和异养细菌的草图基因组。进行了比较宏转录组学分析，以检查优势厌氧氨氧化菌 Candidatus Kuenenia stuttgartiensis 和异养细菌的基因表达，以更好地了解它们的潜在相互作用。上清液中发现的变形菌门相关细菌在产生生长所需的次生代谢物钼喋呤辅因子和叶酸方面非常活跃。此外，Candidatus Kuenenia 基因组中 hzsA 和 CO 固定基因的表达水平显著升高，表明在复苏过程中连续添加厌氧氨氧化上清液后，厌氧氨氧化菌可能更活跃且生长更快。复苏处理脉冲实验证实，连续添加上清液是快速复苏冷冻保存的 anammox 菌的有效方法。我们的发现提供了在冷冻保存的 anammox 菌快速复苏过程中存在交叉喂养的第一个证据。
