Mathieu Elliot, MacPherson Chad W, Belvis Jocelyn, Mathieu Olivier, Robert Véronique, Saint-Criq Vinciane, Langella Philippe, Tompkins Thomas A, Thomas Muriel
Micalis Institute, AgroParisTech, INRAE, Université Paris-Saclay, 78350 Jouy-en-Josas, France.
Rosell Institute for Microbiome and Probiotics, Lallemand Health Solutions Inc., Montreal, QC H4P 2R2, Canada.
Microorganisms. 2020 Jul 22;8(8):1094. doi: 10.3390/microorganisms8081094.
The microbiota of the mouth disperses into the lungs, and both compartments share similar phyla. Considering the importance of the microbiota in the maturation of the immunity and physiology during the first days of life, we hypothesized that primo-colonizing bacteria of the oral cavity may induce immune responses in bronchial epithelial cells. Herein, we have isolated and characterized 57 strains of the buccal cavity of two human newborns. These strains belong to , , , and genera, with being the most represented. The strains were co-incubated with a bronchial epithelial cell line (BEAS-2B), and we established their impact on a panel of cytokines/chemokines and global changes in gene expression. The strains, which appeared soon after birth, induced a high production of IL-8, suggesting they can trigger inflammation, whereas the strains were less associated with inflammation pathways. The genera , and induced differential profiles of cytokine/chemokine/growth factor and set of genes associated with maturation of morphology. Altogether, our results demonstrate that the microorganisms, primo-colonizing the oral cavity, impact immunity and morphology of the lung epithelial cells, with specific effects depending on the phylogeny of the strains.
口腔微生物群会扩散到肺部，且这两个腔室共享相似的门类。鉴于微生物群在生命最初几天免疫和生理成熟过程中的重要性，我们推测口腔的首批定殖细菌可能会在支气管上皮细胞中诱导免疫反应。在此，我们分离并鉴定了两名人类新生儿口腔中的57株菌株。这些菌株属于 、 、 、 和 属，其中 属占比最大。将这些菌株与支气管上皮细胞系（BEAS-2B）共同培养，我们确定了它们对一组细胞因子/趋化因子以及基因表达整体变化的影响。出生后不久出现的 菌株诱导产生大量白细胞介素-8，表明它们可引发炎症，而 菌株与炎症途径的关联较小。 、 和 属诱导了细胞因子/趋化因子/生长因子的不同表达谱以及与形态成熟相关的基因集。总之，我们的结果表明，首批定殖于口腔的微生物会影响肺上皮细胞的免疫和形态，其具体影响取决于菌株的系统发育。
