Lee Hyoban, Youn Hyungjun, Hwang Ahreum, Lee Hyunsoo, Park Jeong Young, Kim Weon, Yoo Youngdong, Ban Changill, Kang Taejoon, Kim Bongsoo
Department of Chemistry, KAIST, Daejeon 34141, Korea.
Department of Chemistry, POSTECH, Pohang 37673, Korea.
Nanomaterials (Basel). 2020 Jul 18;10(7):1402. doi: 10.3390/nano10071402.
Well-ordered bioreceptors on atomically flat Au surfaces can be a high-performance biosensor. Cardiac troponin I proteins (cTnIs) have been regarded as a specific biomarker for acute myocardial infarction (AMI). Here, we report the accurate detection of cTnIs using an aptamer-immobilized Au nanoplate platform. The single-crystalline and atomically flat Au nanoplate was characterized by atomic force microscopy. For the precise detection of cTnI, we immobilized an aptamer that can strongly bind to cTnI onto an atomically flat Au nanoplate. Using the aptamer-immobilized Au nanoplate, cTnIs were successfully detected at a concentration of 100 aM (2.4 fg/mL) in buffer solution. Furthermore, cTnIs in serum could be identified at a concentration of 100 fM (2.4 pg/mL). The total assay time was ~7 h. Importantly, the aptamer-immobilized Au nanoplate enabled us to diagnose AMI patients accurately, suggesting the potential of the present method in the diagnosis of AMI.
原子级平整的金表面上排列有序的生物受体可以成为一种高性能的生物传感器。心肌肌钙蛋白I（cTnI）一直被视为急性心肌梗死（AMI）的特异性生物标志物。在此，我们报告了使用固定有适配体的金纳米片平台对cTnI进行准确检测。通过原子力显微镜对单晶且原子级平整的金纳米片进行了表征。为了精确检测cTnI，我们将能与cTnI紧密结合的适配体固定在原子级平整的金纳米片上。使用固定有适配体的金纳米片，在缓冲溶液中成功检测到浓度为100 aM（2.4 fg/mL）的cTnI。此外，血清中的cTnI在浓度为100 fM（2.4 pg/mL）时也能够被识别。总检测时间约为7小时。重要的是，固定有适配体的金纳米片使我们能够准确诊断AMI患者，表明本方法在AMI诊断中的潜力。
