Department of Chemistry , KAIST , Daejeon 34141 , Korea.
Department of Nanobiotechnology, KRIBB School of Biotechnology , UST , Daejeon 34113 , Korea.
ACS Appl Mater Interfaces. 2018 Nov 7;10(44):37829-37834. doi: 10.1021/acsami.8b13180. Epub 2018 Oct 26.
Multivalent immunoprobes can improve the sensitivity of biosensors because increased valency can strengthen the binding affinity between the receptor and target biomolecules. Here, we report surface-enhanced Raman scattering (SERS)-based immunoassays using multivalent antibody-conjugated nanoparticles (NPs) for the first time. Multivalent antibodies were generated through the ligation of Fab fragments fused with Fc-binding peptides to immunoglobulin G. This fabrication method is easy and fast because of the elimination of heterologous protein expression, high degrees of antibody modifications, and covalent chemical ligation steps. We constructed multivalent antibody-NP conjugates (MANCs) and employed them as SERS immunoprobes. MANCs improved the sensitivity of SERS-based immunoassays by 100 times compared to standard antibody-NP conjugates. MANCs will increase the feasibility of practical SERS-based immunoassays.
多价免疫探针可以提高生物传感器的灵敏度，因为增加价数可以增强受体和目标生物分子之间的结合亲和力。在这里，我们首次报道了基于表面增强拉曼散射（SERS）的多价抗体偶联纳米粒子（NPs）免疫分析。多价抗体是通过将 Fab 片段与 Fc 结合肽融合来制备的，从而连接到免疫球蛋白 G 上。由于消除了异源蛋白表达、高抗体修饰程度和共价化学连接步骤，这种制备方法既简单又快速。我们构建了多价抗体-NP 缀合物（MANC），并将其用作 SERS 免疫探针。与标准抗体-NP 缀合物相比，MANC 将基于 SERS 的免疫分析的灵敏度提高了 100 倍。MANC 将提高实际基于 SERS 的免疫分析的可行性。
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