Prevalence of (ISKNV), Nervous Necrosis Virus (NNV) and Ectoparasites in Juvenile spp. Farmed in Aceh, Indonesia.

A cross-sectional survey was used to estimate the prevalence of infections with the (ISKNV, ), nervous necrosis virus (NNV, ), and infestations with ectoparasites during the rainy season in juvenile grouper ( spp.) farmed in Aceh, Indonesia. The survey was intended to detect aquatic pathogens present at 10% prevalence with 95% confidence, assuming 100% sensitivity and specificity using a sample size of 30 for each diagnostic test. Eight populations of grouper from seven farms were sampled. Additional targeted sampling was conducted for populations experiencing high mortality. Infection with NNV was detected at all farms with seven of the eight populations being positive. The apparent prevalence for NNV ranged from 0% (95% CI: 0-12) to 73% (95% CI: 54-88). All of the fish tested from the targeted samples (Populations 9 and 10) were positive for NNV and all had vacuolation of the brain and retina consistent with viral nervous necrosis (VNN). Coinfections with ISKNV were detected in five populations, with the highest apparent prevalence being 13% (95% CI: 4-31%). sp., and sp. were detected at three farms, with 66% to 100% of fish being infested. Hybrid grouper sourced from a hatchery were 5.4 and 24.9 times more likely to have a NNV infection and a higher parasite load compared to orange-spotted grouper collected from the wild ( < 0.001). This study found that VNN remains a high-impact disease in grouper nurseries in Aceh, Indonesia.