Beaupré Vickie, Boucher Nathalie, Desgagné-Penix Isabel
Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, Trois-Rivières, QC G8Z 4M3, Canada.
Pharmaceutical plant, Devonian Health Group, Montmagny, QC G5V 4T1, Canada.
Biomedicines. 2020 Jul 16;8(7):219. doi: 10.3390/biomedicines8070219.
The anti-inflammatory and antioxidant role of Thykamine, a botanical extract of thylakoides obtained from spinach leaves, has been investigated in animal and cellular models. The oxidative properties have been proven by inhibiting NO production (>98%) in J774A.1 cells and by protecting a linoelic acid emulsion subjected to lipid peroxidation caused by AAPH. Thykamine injected intraperitoneally to rats reduced the inflammatory process of (TNBS)-induced colitis and carrageenan-induced paw edema. As neutrophils are the first cells to migrate to inflammatory sites, the influence of Thykamine on the primary neutrophil functions were studied. Thykamine dose-dependent reduced neutrophil chemiotaxis, phagocytosis, and degranulation. No change in the release of LDH by neutrophils on Thykamine was recorded. Thykamine inhibited by 85% the neutrophil production of O. A superoxide recovery activity was observed on a zymography demonstrating a SOD-like enzyme on Thykamine extracts. Spontaneous fluorescence provided by carotenoid and chlorophyll pigments (488/675 nm) detected Thykamine on the surface, in the cytoplasm (mainly central where Golgi are present) and weakly in the nucleus of neutrophils. The results argue that SOD and pigments found in Thykamine are part of its antioxidant and anti-inflammatory properties shown in in vivo and in vitro models of inflammation.
类囊体胺是一种从菠菜叶中提取的类囊体植物提取物，其抗炎和抗氧化作用已在动物和细胞模型中进行了研究。通过抑制J774A.1细胞中一氧化氮的产生（>98%）以及保护受2,2'-偶氮二异丁腈引起的脂质过氧化作用的亚油酸乳液，证实了其氧化特性。腹腔注射类囊体胺可减轻大鼠由三硝基苯磺酸诱导的结肠炎和角叉菜胶诱导的爪肿胀的炎症过程。由于中性粒细胞是最早迁移到炎症部位的细胞，因此研究了类囊体胺对中性粒细胞主要功能的影响。类囊体胺剂量依赖性地降低了中性粒细胞的趋化性、吞噬作用和脱颗粒作用。未记录到类囊体胺对中性粒细胞释放乳酸脱氢酶的影响。类囊体胺抑制了中性粒细胞85%的超氧阴离子产生。在酶谱分析中观察到超氧化物恢复活性，表明类囊体胺提取物上存在一种超氧化物歧化酶样酶。类胡萝卜素和叶绿素色素提供的自发荧光（488/675 nm）在中性粒细胞表面、细胞质（主要在存在高尔基体的中央）以及细胞核中微弱地检测到类囊体胺。结果表明，类囊体胺中发现的超氧化物歧化酶和色素是其在体内和体外炎症模型中显示的抗氧化和抗炎特性的一部分。
