Department of Medical Microbiology and Infectious Diseases, Manitoba Centre for Proteomics & Systems Biology, Room 799, University of Manitoba, 715 McDermot Avenue, Winnipeg, MB R3E 3P4, Canada.
Children's Hospital Research Institute of Manitoba, Room 513, John Buhler Research Centre, 715 McDermot Avenue, Winnipeg, MB R3E 3P4, Canada.
Viruses. 2020 Jul 17;12(7):771. doi: 10.3390/v12070771.
The zika virus (ZIKV) is a neurotropic virus that causes congenital abnormalities in babies when they are infected in utero. Some studies have reported these congenital abnormalities result from ZIKV attacking neural progenitor cells within the brain which differentiate into neurons, oligodendrocytes, and astrocytes. Each of these glial cells play important roles during development of the fetal brain. In addition to ZIKV-induced congenital abnormalities, infected patients experience gastrointestinal complications. There are presently no reports investigating the role of this virus at the proteomic level in gastrointestinal associated cells, so we conducted an in vitro proteomic study of ZIKV-induced changes in Caco-2, a colon-derived human cell line which is known to be permissive to ZIKV infection. We used SomaScan, a new aptamer-based proteomic tool to identify host proteins that are dysregulated during ZIKV infection at 12, 24, and 48 h post-infection. Bioinformatic analyses predicted that dysregulation of differentially-regulated host proteins results in various gastrointestinal diseases. Validation of the clinical relevance of these promising protein targets will add to the existing knowledge of ZIKV biology. These potential proteins may be useful targets towards the development of therapeutic interventions.
寨卡病毒（ZIKV）是一种神经嗜性病毒，当婴儿在子宫内感染时，会导致婴儿先天畸形。一些研究报告称，这些先天畸形是由于 ZIKV 攻击大脑内的神经祖细胞，这些细胞分化为神经元、少突胶质细胞和星形胶质细胞。这些神经胶质细胞在胎儿大脑发育过程中都起着重要作用。除了 ZIKV 引起的先天畸形外，感染的患者还会出现胃肠道并发症。目前尚无报道研究这种病毒在胃肠道相关细胞中的蛋白质组学水平的作用，因此我们对 Caco-2 进行了体外蛋白质组学研究，Caco-2 是一种源自结肠的人细胞系，已知其对 ZIKV 感染具有易感性。我们使用了 SomaScan，一种新的基于适配体的蛋白质组学工具，来鉴定在感染 ZIKV 后 12、24 和 48 小时时发生失调的宿主蛋白。生物信息学分析预测，差异调节的宿主蛋白失调会导致各种胃肠道疾病。这些有前途的蛋白靶点的临床相关性的验证将增加对 ZIKV 生物学的现有认识。这些潜在的蛋白质可能是开发治疗干预措施的有用靶点。
