Rupa Esrat Jahan, Arunkumar Lakshminarayanan, Han Yaxi, Kang Jong Pyo, Ahn Jong Chan, Jung Seok-Kyu, Kim Mia, Kim Jong Yun, Yang Deok-Chun, Lee Gyong Jai
Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University, Yongin-si 17104, Gyeonggi-do, Korea.
Department of Cardiovascular and Neurologic Diseases, College of Korean Medicine, Kyung Hee University, Seoul 100011, Korea.
Materials (Basel). 2020 Jul 17;13(14):3197. doi: 10.3390/ma13143197.
is a versatile plant that has been used as a herbal medicine due to its various useful medicinal effects. To protect its active component from biological stress and increase its drug efficacy as well as drug bioavailability, nanoemulsion was prepared. zinc oxide nanoparticles (-ZnO NPs) were synthesized using the plant extract via the co-precipitation method and loaded with active indole-3-carbinol for nanoemulsion formulation using the ultrasonication process. Field emission transmission electron microscope revealed the flower shape of the indole-3-carbinol zinc oxide nanoemulsion (-ZnO-I3C-NE). In contrast, -ZnO NPs showed a spheroid shape that coincides agreeably with field emission electron scanning microscope. The hydrodynamic sizes by dynamic light scattering are about 65 ± 3 nm and 239.6 ± 6 nm and the crystallite sizes from X-ray diffraction are 11.52 nm and 16.07 nm for -ZnO NPs and -ZnO-I3C-NE, respectively. In vitro analysis revealed the cytotoxicity of -ZnO-I3C-NE against a human lung cancer cell line (A549) at 12.5 µg/mL as well as reactive oxygen species (ROS) production. The -ZnO-I3C-NE-induced ROS generation level was higher than that of -ZnO NPs and free indole-3-carbinol. The synergistic effect of -ZnO and indole-3-carbinol indicates -ZnO-I3C-NE as a potential candidate for future lung cancer drug and could be scope for functional food.
是一种用途广泛的植物，因其多种有益的药用功效而被用作草药。为了保护其活性成分免受生物应激，并提高其药物疗效以及药物生物利用度，制备了纳米乳液。通过共沉淀法使用植物提取物合成了氧化锌纳米颗粒（-ZnO NPs），并使用超声处理过程将活性吲哚 - 3 - 甲醇负载到纳米乳液配方中。场发射透射电子显微镜揭示了吲哚 - 3 - 甲醇氧化锌纳米乳液（-ZnO-I3C-NE）的花状形态。相比之下，-ZnO NPs呈现出与场发射电子扫描显微镜相符的球状形态。通过动态光散射测得的流体动力学尺寸对于 -ZnO NPs和 -ZnO-I3C-NE分别约为65±3 nm和239.6±6 nm，并且通过X射线衍射测得的微晶尺寸分别为11.52 nm和16.07 nm。体外分析揭示了 -ZnO-I3C-NE在12.5 µg/mL时对人肺癌细胞系（A549）的细胞毒性以及活性氧（ROS）的产生。-ZnO-I3C-NE诱导的ROS生成水平高于 -ZnO NPs和游离吲哚 - 3 - 甲醇。-ZnO和吲哚 - 3 - 甲醇的协同作用表明 -ZnO-I3C-NE作为未来肺癌药物的潜在候选物，并且可能成为功能性食品的研究范围。
