Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, China.
Department of Biotechnology, College of Life Science, Kyung Hee University, Yongin-si 17104, Gyeonggi-do, Republic of Korea.
Molecules. 2023 Jan 16;28(2):906. doi: 10.3390/molecules28020906.
The unique and tailorable physicochemical features of zinc oxide nanoparticles (ZnO-NPs) synthesized from green sources make them attractive for use in cancer treatment. Hydroponic-cultured ginseng-root-synthesized ZnO-NPs (HGRCm-ZnO NPs) were coated with O-carboxymethyl chitosan (CMC) polymer, which stabilized and enhanced the biological efficacy of the nanoparticles. Nanoparticles were characterized by X-ray diffraction (XRD), UV-Vis spectroscopy, transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FT-IR), and energy-dispersive X-ray spectroscopy (EDS). The flower-shaped nanoparticles were crystalline in nature with a particle size of 28 nm. To evaluate if these NPs had anti-lung cancer activity, analysis was performed on a human lung carcinoma cell line (A549). HGRCm-ZnO nanoparticles showed less toxicity to normal keratinocytes (HaCaTs), at concentrations up to 20 µg/mL, than A549 cancer cells. Additionally, these NPs showed dose-dependent colony formation and cell migration inhibition ability, which makes them more promising for lung cancer treatment. Additionally, Hoechst and propidium iodide dye staining also confirmed that the NP formulation had apoptotic activity in cancer cells. Further, to evaluate the mechanism of cancer cell death via checking the gene expression, HGRCm ZnO NPs upregulated the and and expression levels but downregulated expression, indicating that the nanoformulation induced mitochondrial-mediated apoptosis. Moreover, these preliminary results suggest that HGRCm ZnO NPs can be a potential candidate for future lung cancer treatment.
由绿色来源合成的氧化锌纳米粒子（ZnO-NPs）具有独特且可定制的物理化学特性，这使得它们在癌症治疗中具有吸引力。水培人参根合成的氧化锌纳米粒子（HGRCm-ZnO NPs）被 O-羧甲基壳聚糖（CMC）聚合物包裹，这稳定并增强了纳米粒子的生物功效。纳米粒子通过 X 射线衍射（XRD）、紫外-可见分光光度法、透射电子显微镜（TEM）、傅里叶变换红外光谱（FT-IR）和能谱（EDS）进行了表征。花状纳米粒子具有结晶性质，粒径为 28nm。为了评估这些 NPs 是否具有抗肺癌活性，对人肺癌细胞系（A549）进行了分析。HGRCm-ZnO 纳米粒子在高达 20μg/mL 的浓度下对正常角质形成细胞（HaCaTs）的毒性小于 A549 癌细胞。此外，这些 NPs 表现出剂量依赖性的集落形成和细胞迁移抑制能力，这使得它们在肺癌治疗中更有前途。此外，Hoechst 和碘化丙啶染料染色也证实了 NP 制剂在癌细胞中具有凋亡活性。此外，为了评估通过检查基因表达导致癌细胞死亡的机制，HGRCm ZnO NPs 上调了 和 以及 的表达水平，但下调了 的表达，表明纳米制剂诱导了线粒体介导的细胞凋亡。此外，这些初步结果表明，HGRCm ZnO NPs 可以成为未来肺癌治疗的潜在候选药物。
