Vernadsky Institute of Geochemistry and Analytical Chemistry, Kosygin St. 19, 119991, Moscow, Russian Federation.
Chair of Analytical Chemistry, Faculty of Chemistry, Warsaw University of Technology, Noakowskiego St. 3, 00-664, Warsaw, Poland.
J Pharm Biomed Anal. 2020 Sep 10;189:113479. doi: 10.1016/j.jpba.2020.113479. Epub 2020 Jul 16.
The potential of iron oxide-based nanoparticles (IONs) as theranostic agents is believed to be in a great part due to non-invasive diagnosis and therapeutic applications. However, there is still a lack of well-recognized methodology to assess bioresistance, hypotoxicity, reactivity toward pertinent biomolecules, as well as an eventual dose of IONs as prerequisites for their clinical use. In this study, we demonstrate how application of high-resolution ICP-MS in combination with conventional ultrafiltration can address these important issues in a simple and high-throughput way. Based upon interference-free and sensitive measurements of iron and sulfur isotopes ensured by sector-field ICP-MS mode, the comparative testing of a series of novel IONs modified by PEG or PEG and an ionic liquid, was performed. Satisfactory stability (less than 1 % of soluble Fe), minor toxicity (by virtue of releasing a free iron) and transit into bioconjugates in human serum, different in speed, proved the prospective of the tested IONs for in-depth preclinical development.
氧化铁基纳米粒子（IONs）作为治疗诊断双效试剂的潜力很大程度上归因于其非侵入性的诊断和治疗应用。然而，目前仍然缺乏公认的方法来评估生物抗性、低毒性、对相关生物分子的反应性以及 IONs 的最终剂量，这些都是其临床应用的前提条件。在本研究中，我们展示了如何应用高分辨率 ICP-MS 结合常规超滤技术，以简单、高通量的方式解决这些重要问题。基于扇形场 ICP-MS 模式确保的铁和硫同位素的无干扰和灵敏测量，对一系列经过 PEG 或 PEG 和离子液体修饰的新型 IONs 进行了对比测试。在人血清中，IONs 表现出令人满意的稳定性（小于 1%的可溶性铁）、较小的毒性（由于释放出游离铁）和转变成生物缀合物，其转化速度不同，这证明了所测试的 IONs 具有深入的临床前开发前景。
