Chair of Analytical Chemistry, Faculty of Chemistry, Warsaw University of Technology, Noakowskiego St. 3, 00-664 Warsaw, Poland.
Centre for Advanced Materials and Technologies CEZAMAT PW, Warsaw University of Technology, Poleczki St. 19, 02-822 Warsaw, Poland.
Int J Mol Sci. 2022 Jan 19;23(3):1088. doi: 10.3390/ijms23031088.
Progress toward translating superparamagnetic iron oxide nanoparticles (SPIONs) with specific diagnostic and therapeutic properties for clinical applications depends on developing and implementing appropriate methodologies that would allow in-depth characterizations of their behavior in a real biological environment. Herein, we report a versatile approach for studying interactions between SPIONs and proteins using single-particle inductively coupled plasma tandem mass spectrometry. By monitoring the changes in the size distribution upon exposure to human serum, the formation of stable protein corona is revealed, accompanied by particle disaggregation.
实现具有特定诊断和治疗性能的超顺磁性氧化铁纳米粒子（SPIONs）向临床应用转化的进展取决于开发和实施适当的方法，这些方法将允许深入了解它们在真实生物环境中的行为。在这里，我们报告了一种使用单颗粒电感耦合等离子体质谱串联质谱法研究 SPIONs 与蛋白质相互作用的通用方法。通过监测暴露于人血清后粒径分布的变化，揭示了稳定的蛋白质冠的形成，同时伴随着颗粒解聚。
