Jung Eun-Joo, Lee Ju-Han, Min Byung-Wook, Kim Young-Sik, Choi Jong Sang
Department of Pathology, Korea University College of Medicine, Seoul, South Korea.
Indian J Pathol Microbiol. 2011 Jan-Mar;54(1):32-6. doi: 10.4103/0377-4929.77320.
The over expression of fascin, extracellular matrix metalloproteinase inducer (EMMPRIN), and ezrin proteins has been associated with poor prognosis in various carcinomas and sarcomas. However, very few studies have reported the relationship between the expression of fascin, EMMPRIN, and ezrin proteins and the clinico-pathologic parameters of colorectal carcinomas.
The aim was to investigate the relationship between fascin, EMMPRIN, and ezrin proteins in colorectal adenocarcinomas and their correlation with clinico-pathologic parameters.
The expression of fascin, EMMPRIN, and ezrin proteins was studied in 210 colorectal adenocarcinoma patients through immunohistochemical staining.
Immunohistochemical staining by the avidin-biotin peroxidase method was done. The scoring of each protein expression was done and divided into three groups (negative, low-, and high-expression groups).
A chi-square test, and Kendall's tau-b correlation test were used for comparing. Survival analysis was performed using the Kaplan-Meier method with log-rank tests and the Cox proportional hazard model.
The percentages of the high-expression group of fascin, EMMPRIN, and ezrin proteins in colorectal adenocarcinomas were 24%, 73%, and 62%, respectively. Weak positive correlations were observed among these protein expressions. An increased expression of the fascin protein was significantly associated with advanced tumor depth and shorter survival times, and a high expression of fascin protein was an independent prognostic factor in univariate and multivariate survival analyses. EMMPRIN and ezrin protein expressions were not associated with the clinico-pathologic parameters.
The high expression of fascin protein may be an unfavorable prognostic marker for individual colorectal cancer patients.
肌动蛋白、细胞外基质金属蛋白酶诱导因子（EMMPRIN）和埃兹蛋白的过表达与多种癌和肉瘤的预后不良相关。然而，很少有研究报道肌动蛋白、EMMPRIN和埃兹蛋白的表达与结直肠癌临床病理参数之间的关系。
研究结直肠腺癌中肌动蛋白、EMMPRIN和埃兹蛋白之间的关系及其与临床病理参数的相关性。
通过免疫组织化学染色研究210例结直肠腺癌患者中肌动蛋白、EMMPRIN和埃兹蛋白的表达。
采用抗生物素蛋白-生物素过氧化物酶法进行免疫组织化学染色。对每种蛋白质表达进行评分并分为三组（阴性、低表达和高表达组）。
采用卡方检验和肯德尔tau-b相关检验进行比较。使用Kaplan-Meier法和对数秩检验以及Cox比例风险模型进行生存分析。
结直肠腺癌中肌动蛋白、EMMPRIN和埃兹蛋白高表达组的百分比分别为24%、73%和62%。这些蛋白质表达之间观察到弱正相关。肌动蛋白表达增加与肿瘤深度进展和生存时间缩短显著相关，在单变量和多变量生存分析中，肌动蛋白高表达是独立的预后因素。EMMPRIN和埃兹蛋白表达与临床病理参数无关。
肌动蛋白高表达可能是个别结直肠癌患者的不良预后标志物。
