Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University, Shenyang 110819, People's Republic of China.
Institute of Health Sciences, China Medical University, Shenyang 110122, People's Republic of China.
Fitoterapia. 2020 Oct;146:104692. doi: 10.1016/j.fitote.2020.104692. Epub 2020 Jul 25.
Physalis pubescens L. is a medicinal plant widely cultivated in northeast of China. Investigation on the extract of P. pubescens fruit led to the isolation and identification of four new withanolides, namely, physapubescins J-M (1, 2, 4 and 5), together with four known analogues (3, 6-8) and fifteen other compounds. Their structures were elucidated on the basis of comprehensive NMR, MS, and ECD spectroscopic data analysis. Among isolates, physapubescin J (1) contained an unusual sulphide linkage, and four withanolides (3, 5, 7 and 8) showed anti-inflammatory potential in LPS-induced RAW264.7 cells. This study supports P. pubescens fruit could be a valuable source of withanolides. Further studies to investigate anti-inflammatory activities of isolated withanolides using in vivo models are warranted.
地稍瓜是一种广泛种植在中国东北的药用植物。对地稍瓜果实提取物的研究导致了四个新的羽扇豆烷类化合物的分离和鉴定，分别为 physapubescins J-M（1、2、4 和 5），以及四个已知的类似物（3、6-8）和其他十五个化合物。它们的结构是基于全面的 NMR、MS 和 ECD 光谱数据分析来阐明的。在分离出的化合物中，physapubescin J（1）含有一个不寻常的硫化物键，四个羽扇豆烷类化合物（3、5、7 和 8）在 LPS 诱导的 RAW264.7 细胞中表现出抗炎潜力。本研究支持地稍瓜果实可能是羽扇豆烷类化合物的有价值来源。进一步研究使用体内模型来研究分离出的羽扇豆烷类化合物的抗炎活性是有必要的。
