Lin Ru, Guan Yu-Zhou, Li Rui-Jun, Xu Xiao-Ming, Luo Jian-Guang, Kong Ling-Yi
State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing, 210009, P. R. China.
Chem Biodivers. 2016 Jul;13(7):884-90. doi: 10.1002/cbdv.201500282. Epub 2016 Jul 2.
Four new 13,14-seco-withanolides, minisecolides A - D (1 - 4), together with three known analogues 5 - 7, were isolated from the whole plants of Physalis minima. The structures of new compounds were determined on the basis of spectroscopic analysis, including (1) H-, (13) C-NMR, 2D-NMR (HMBC, HSQC, ROESY), and HR-ESI-MS. Evaluation of all isolates for their inhibitory effects on nitric oxide (NO) production was conducted on lipopolysaccaride-activated RAW264.7 macrophages. Compounds 2, 3, 5, and 6 showed inhibitory activities, especially for compound 5 with IC50 value of 3.87 μm.
从小酸浆全草中分离得到4个新的13,14-断-维烷型内酯类化合物，即小断内酯A - D（1 - 4），以及3个已知类似物5 - 7。通过光谱分析，包括¹H-、¹³C-NMR、二维NMR（HMBC、HSQC、ROESY）和高分辨电喷雾电离质谱，确定了新化合物的结构。在脂多糖激活的RAW264.7巨噬细胞上评估了所有分离物对一氧化氮（NO）产生的抑制作用。化合物2、3、5和6表现出抑制活性，尤其是化合物5，其IC50值为3.87μm。
