State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, 300350, People's Republic of China.
College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Republic of Korea.
Phytochemistry. 2022 Oct;202:113301. doi: 10.1016/j.phytochem.2022.113301. Epub 2022 Jun 30.
Eight undescribed and two known withanolides were obtained from the aerial parts of Physalis minima. The structures of these compounds were defined by spectroscopic methods including 1D and 2D NMR, HRESIMS, and electronic circular dichroism (ECD) data analysis. Physminin E was elucidated to be a rare 13,14-seco-withanolide. Inhibitory effects of these compounds on nitric oxide (NO) production were evaluated by using LPS-activated RAW264.7 macrophages, and physminin C was shown to be the most active with an IC value of 3.5 μM. The further mechanistic analysis of NO inhibition was performed by molecular docking and Western blotting.
从酸浆果的地上部分中分离得到了 8 个未见报道的和 2 个已知的醉茄内酯。这些化合物的结构通过包括 1D 和 2D NMR、HRESIMS 和电子圆二色性（ECD）数据分析在内的光谱方法来确定。Physminin E 被阐明为一种罕见的 13,14-去甲醉茄内酯。通过 LPS 激活的 RAW264.7 巨噬细胞评估了这些化合物对一氧化氮（NO）生成的抑制作用，结果表明 physminin C 具有最强的活性，IC 值为 3.5 μM。通过分子对接和 Western blot 进一步进行了 NO 抑制的机制分析。
