Kamb A, Tseng-Crank J, Tanouye M A
Division of Biology, California Institute of Technology, Pasadena 91125.
Neuron. 1988 Jul;1(5):421-30. doi: 10.1016/0896-6273(88)90192-4.
K+ channels are known through electrophysiology and pharmacology to be an exceptionally diverse group of channels. Molecular studies of the Shaker (Sh) locus in Drosophila have provided the first glimpse of K+ channel structure. The sequences of several Sh cDNA clones have been reported; none are identical. We have isolated and examined 18 additional Sh cDNAs in an attempt to understand the origin, extent, and significance of the variability. The diversity is extensive: we have already identified cDNAs representing at least nine distinct types, and Sh could potentially encode 24 or more products. This diversity, however, fits a simple pattern in which variable 3' and 5' ends are spliced onto a central constant region to yield different cDNA types. These different Sh cDNAs encode proteins with distinct structural features.
通过电生理学和药理学研究可知，钾离子通道是一类极其多样的通道。对果蝇中振子（Sh）位点的分子研究首次揭示了钾离子通道的结构。已报道了几个Sh cDNA克隆的序列；但没有一个是相同的。我们分离并检测了另外18个Sh cDNA，试图了解这种变异性的起源、程度和意义。这种多样性很广泛：我们已经鉴定出代表至少九种不同类型的cDNA，并且Sh可能潜在地编码24种或更多的产物。然而，这种多样性符合一种简单的模式，即可变的3'和5'末端被剪接到一个中央恒定区域上，以产生不同的cDNA类型。这些不同的Sh cDNA编码具有不同结构特征的蛋白质。
