果蝇的多动基因座编码一种与醛糖酮还原酶超家族相关的钾通道β亚基。
A potassium channel beta subunit related to the aldo-keto reductase superfamily is encoded by the Drosophila hyperkinetic locus.
作者信息
Chouinard S W, Wilson G F, Schlimgen A K, Ganetzky B
机构信息
Laboratory of Genetics, University of Wisconsin, Madison 53706, USA.
出版信息
Proc Natl Acad Sci U S A. 1995 Jul 18;92(15):6763-7. doi: 10.1073/pnas.92.15.6763.
Abstract
Genetic and physiological studies of the Drosophila Hyperkinetic (Hk) mutant revealed defects in the function or regulation of K+ channels encoded by the Shaker (Sh) locus. The Hk polypeptide, determined from analysis of cDNA clones, is a homologue of mammalian K+ channel beta subunits (Kv beta). Coexpression of Hk with Sh in Xenopus oocytes increases current amplitudes and changes the voltage dependence and kinetics of activation and inactivation, consistent with predicted functions of Hk in vivo. Sequence alignments show that Hk, together with mammalian Kv beta, represents an additional branch of the aldo-keto reductase superfamily. These results are relevant to understanding the function and evolutionary origin of Kv beta.
摘要
对果蝇“运动过强”（Hk）突变体的遗传学和生理学研究揭示了由“振子”（Sh）基因座编码的钾离子通道在功能或调节方面的缺陷。通过对cDNA克隆的分析确定，Hk多肽是哺乳动物钾离子通道β亚基（Kvβ）的同源物。在非洲爪蟾卵母细胞中，Hk与Sh共同表达会增加电流幅度，并改变激活和失活的电压依赖性及动力学，这与Hk在体内的预测功能一致。序列比对表明，Hk与哺乳动物Kvβ一起，代表了醛酮还原酶超家族的一个额外分支。这些结果对于理解Kvβ的功能和进化起源具有重要意义。
相似文献
1
A potassium channel beta subunit related to the aldo-keto reductase superfamily is encoded by the Drosophila hyperkinetic locus.果蝇的多动基因座编码一种与醛糖酮还原酶超家族相关的钾通道β亚基。
Proc Natl Acad Sci U S A. 1995 Jul 18;92(15):6763-7. doi: 10.1073/pnas.92.15.6763.
2
Auxiliary Hyperkinetic beta subunit of K+ channels: regulation of firing properties and K+ currents in Drosophila neurons.钾离子通道辅助超动力β亚基：果蝇神经元放电特性和钾电流的调节
J Neurophysiol. 1999 May;81(5):2472-84. doi: 10.1152/jn.1999.81.5.2472.
3
In vivo functional role of the Drosophila hyperkinetic beta subunit in gating and inactivation of Shaker K+ channels.果蝇超动力β亚基在Shaker钾离子通道门控和失活中的体内功能作用。
Biophys J. 1996 Dec;71(6):3167-76. doi: 10.1016/S0006-3495(96)79510-3.
4
Cloning and functional expression of a Shaker-related voltage-gated potassium channel gene from Schistosoma mansoni (Trematoda: Digenea).曼氏血吸虫（吸虫纲：复殖目）中一个与Shaker相关的电压门控钾通道基因的克隆及功能表达
Parasitology. 1995 Feb;110 ( Pt 2):171-80. doi: 10.1017/s0031182000063939.
5
Molecular and functional characterization of a rat brain Kv beta 3 potassium channel subunit.大鼠脑Kvβ3钾通道亚基的分子与功能特性
FEBS Lett. 1995 Dec 27;377(3):383-9. doi: 10.1016/0014-5793(95)01377-6.
6
Interaction of the K channel beta subunit, Hyperkinetic, with eag family members.钾通道β亚基Hyperkinetic与eag家族成员的相互作用。
J Biol Chem. 1998 Mar 13;273(11):6389-94. doi: 10.1074/jbc.273.11.6389.
7
Apoptotic proteins Reaper and Grim induce stable inactivation in voltage-gated K+ channels.凋亡蛋白Reaper和Grim可诱导电压门控钾通道发生稳定失活。
Proc Natl Acad Sci U S A. 1998 Sep 29;95(20):11703-8. doi: 10.1073/pnas.95.20.11703.
8
K+ current diversity is produced by an extended gene family conserved in Drosophila and mouse.钾离子电流多样性由果蝇和小鼠中保守的一个扩展基因家族产生。
Science. 1990 May 4;248(4955):599-603. doi: 10.1126/science.2333511.
9
Regulation of the voltage-gated K+ channel KCNA10 by KCNA4B, a novel beta-subunit.新型β亚基KCNA4B对电压门控钾通道KCNA10的调控
Am J Physiol Renal Physiol. 2002 Jul;283(1):F142-9. doi: 10.1152/ajprenal.00258.2001.
10
Cloning of a human cDNA expressing a high voltage-activating, TEA-sensitive, type-A K+ channel which maps to chromosome 1 band p21.一个表达高电压激活、对四乙铵敏感的A型钾通道的人类cDNA的克隆，该通道定位于1号染色体p21带。
J Neurosci Res. 1991 Jul;29(3):401-12. doi: 10.1002/jnr.490290316.
引用本文的文献
1
Deficiency in transmitter release triggers homeostatic transcriptional changes that increase presynaptic excitability.神经递质释放缺陷会引发稳态转录变化，从而增加突触前兴奋性。
Proc Natl Acad Sci U S A. 2025 Aug 5;122(31):e2322714122. doi: 10.1073/pnas.2322714122. Epub 2025 Jul 29.
2
Sleep pressure accumulates in a voltage-gated lipid peroxidation memory.睡眠压力积聚于电压门控脂质过氧化记忆中。
Nature. 2025 May;641(8061):232-239. doi: 10.1038/s41586-025-08734-4. Epub 2025 Mar 19.
3
Kv Channel Ancillary Subunits: Where Do We Go from Here?Kv 通道辅助亚基：我们从何处出发？
Physiology (Bethesda). 2022 Sep 1;37(5):0. doi: 10.1152/physiol.00005.2022.
4
The potassium channel subunit Kβ1 serves as a major control point for synaptic facilitation.钾通道亚基 Kβ1 是突触易化的主要控制点。
Proc Natl Acad Sci U S A. 2020 Nov 24;117(47):29937-29947. doi: 10.1073/pnas.2000790117. Epub 2020 Nov 9.
5
Biochemical and physiological properties of K channel-associated AKR6A (Kvβ) proteins.K 通道相关 AKR6A（Kvβ）蛋白的生化和生理特性。
Chem Biol Interact. 2019 May 25;305:21-27. doi: 10.1016/j.cbi.2019.03.023. Epub 2019 Mar 26.
6
Lithium-Responsive Seizure-Like Hyperexcitability Is Caused by a Mutation in the Voltage-Gated Sodium Channel Gene .锂反应性癫痫样过度兴奋由电压门控钠通道基因突变引起。
eNeuro. 2016 Nov 10;3(5). doi: 10.1523/ENEURO.0221-16.2016. eCollection 2016 Sep-Oct.
7
CRYPTOCHROME-mediated phototransduction by modulation of the potassium ion channel β-subunit redox sensor.通过调节钾离子通道β亚基氧化还原传感器实现的隐花色素介导的光转导。
Proc Natl Acad Sci U S A. 2015 Feb 17;112(7):2245-50. doi: 10.1073/pnas.1416586112. Epub 2015 Feb 2.
8
Hypnotic effects of a novel anti-insomnia formula on Drosophila insomnia model.一种新型抗失眠配方对果蝇失眠模型的催眠作用
Chin J Integr Med. 2016 May;22(5):335-43. doi: 10.1007/s11655-014-1625-1. Epub 2014 Aug 26.
9
Flight and seizure motor patterns in Drosophila mutants: simultaneous acoustic and electrophysiological recordings of wing beats and flight muscle activity.果蝇突变体中的飞行和癫痫发作运动模式：翅膀拍打和飞行肌肉活动的同步声学和电生理记录。
J Neurogenet. 2014 Sep-Dec;28(3-4):316-28. doi: 10.3109/01677063.2014.957827.
10
Automated quantification of locomotion, social interaction, and mate preference in Drosophila mutants.果蝇突变体运动、社交互动和配偶偏好的自动量化
J Neurogenet. 2012 Sep;26(3-4):306-16. doi: 10.3109/01677063.2012.729626. Epub 2012 Oct 29.
本文引用的文献
1
Conversion of Glucose to 2-Keto-l-Gulonate, an Intermediate in l-Ascorbate Synthesis, by a Recombinant Strain of Erwinia citreus.利用重组欧文氏菌（Erwinia citreus）将葡萄糖转化为 2-酮基-l-古洛糖酸，这是 l-抗坏血酸合成的中间产物。
Appl Environ Microbiol. 1988 Jul;54(7):1770-5. doi: 10.1128/aem.54.7.1770-1775.1988.
2
Bacterial morphine dehydrogenase further defines a distinct superfamily of oxidoreductases with diverse functional activities.细菌吗啡脱氢酶进一步定义了一个具有多种功能活性的独特氧化还原酶超家族。
Biochem J. 1994 May 1;299 ( Pt 3)(Pt 3):805-11. doi: 10.1042/bj2990805.
3
Inactivation properties of voltage-gated K+ channels altered by presence of beta-subunit.β亚基的存在改变电压门控钾离子通道的失活特性。
Nature. 1994 May 26;369(6478):289-94. doi: 10.1038/369289a0.
4
Three-dimensional structure of rat liver 3 alpha-hydroxysteroid/dihydrodiol dehydrogenase: a member of the aldo-keto reductase superfamily.大鼠肝脏3α-羟基类固醇/二氢二醇脱氢酶的三维结构：醛酮还原酶超家族的一员。
Proc Natl Acad Sci U S A. 1994 Mar 29;91(7):2517-21. doi: 10.1073/pnas.91.7.2517.
5
Shaker K+ channel beta subunits belong to an NAD(P)H-dependent oxidoreductase superfamily.震颤器钾离子通道β亚基属于一个依赖烟酰胺腺嘌呤二核苷酸（磷酸）的氧化还原酶超家族。
Cell. 1994 Dec 30;79(7):1133-5. doi: 10.1016/0092-8674(94)90004-3.
6
Auxiliary subunits of voltage-gated ion channels.电压门控离子通道的辅助亚基。
Neuron. 1994 Jun;12(6):1183-94. doi: 10.1016/0896-6273(94)90436-7.
7
The behavior of four neurological mutants of Drosophila.果蝇四种神经突变体的行为
Genetics. 1969 Feb;61(2):399-409. doi: 10.1093/genetics/61.2.399.
8
Multiple products of the Drosophila Shaker gene may contribute to potassium channel diversity.果蝇Shaker基因的多种产物可能有助于钾通道的多样性。
Neuron. 1988 Jul;1(5):421-30. doi: 10.1016/0896-6273(88)90192-4.
9
A stable genomic source of P element transposase in Drosophila melanogaster.黑腹果蝇中P因子转座酶的稳定基因组来源。
Genetics. 1988 Mar;118(3):461-70. doi: 10.1093/genetics/118.3.461.
10
Structural similarity of bovine lung prostaglandin F synthase to lens epsilon-crystallin of the European common frog.牛肺前列腺素F合酶与欧洲普通青蛙晶状体ε-晶体蛋白的结构相似性。
Proc Natl Acad Sci U S A. 1988 Jan;85(1):11-5. doi: 10.1073/pnas.85.1.11.
Zotero 插件