Wang Kaijun, Ren Ao, Zheng Mengli, Jiao Jinzhen, Yan Qiongxian, Zhou Chuanshe, Tan Zhiliang
CAS Key Laboratory of Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutrition & Physiology and Metabolism, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, China.
Hunan Co-Innovation Center of Animal Production Safety, CICAPS, Changsha 410128, China.
Animals (Basel). 2020 Jul 24;10(8):1261. doi: 10.3390/ani10081261.
Effects of a high proportion of concentrate in the diet on the ileal microbiota and metabolites in small ruminants are rarely reported. This study was designed to investigate the ileal microbiota and its relationship with host metabolic function in goats and aimed to elucidate the mechanisms involving in the ileal adaptation to a diet containing a high proportion of rice. Sixteen goats were equally divided into two groups and fed a diet with a normal concentrate proportion (NC, 55% concentrate) or a high-concentrate diet (HC, 90% concentrate). Results showed that the HC diet decreased bacterial diversity and elevated the abundance of five genera (___, __group, __group, __group and 1), but reduced the number of Anaerotruncus. Microbial functional potentials indicated that the HC diet activated the pathways related to metabolism of carbohydrate, glycan, lipid and vitamins, but inhibited the pathways associated with cell motility and signal transduction. The activities of amylase and alkaline phosphatase were greater ( < 0.05) in the intestinal digesta of the HC-fed goats. However, there were no differences in the villus height, crypt depth and the ratio of villus height to crypt depth in the ileum between the two groups. These results indicate that the HC diet alters the bacterial community and pathways related to the metabolism of dietary nutrients and cell motility and signal transduction of bacteria in the ileum of goats.
日粮中高比例精料对小反刍动物回肠微生物群和代谢产物的影响鲜有报道。本研究旨在调查山羊回肠微生物群及其与宿主代谢功能的关系，旨在阐明回肠适应高比例稻谷日粮的机制。将16只山羊平均分为两组，分别饲喂正常精料比例日粮（NC，精料比例55%）或高精料日粮（HC，精料比例90%）。结果表明，HC日粮降低了细菌多样性，提高了五个属（___、__组、__组、__组和1）的丰度，但减少了厌氧短杆菌属的数量。微生物功能潜力表明，HC日粮激活了与碳水化合物、聚糖、脂质和维生素代谢相关的途径，但抑制了与细胞运动和信号转导相关的途径。HC组山羊肠道食糜中淀粉酶和碱性磷酸酶的活性更高（<0.05）。然而，两组间回肠绒毛高度、隐窝深度及绒毛高度与隐窝深度之比并无差异。这些结果表明，HC日粮改变了山羊回肠中与膳食营养物质代谢以及细菌细胞运动和信号转导相关的细菌群落和途径。
