Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, 146 Brownlow Hill, Liverpool L3 5RF, UK.
School of Science, Engineering and Environment, G32 Peel Building, University of Salford, Salford M5 4WT, UK.
Ticks Tick Borne Dis. 2020 Sep;11(5):101501. doi: 10.1016/j.ttbdis.2020.101501. Epub 2020 Jun 27.
Trypanosomes have long been recognised as being amongst the most important protozoan parasites of vertebrates, from both medical and veterinary perspectives. Whilst numerous insect species have been identified as vectors, the role of ticks is less well understood. Here we report the isolation and partial molecular characterisation of a novel trypanosome from questing Ixodes ricinus ticks collected in Slovakia. The trypanosome was isolated in tick cell culture and then partially characterised by microscopy and amplification of fragments of the 18S rRNA and 24Sα rDNA genes. Analysis of the resultant sequences suggests that the trypanosome designated as Trypanosoma sp. Bratislava1 may be a new species closely related to several species or strains of trypanosomes isolated from, or detected in, ticks in South America and Asia, and to Trypanosoma caninum isolated from dogs in Brazil. This study highlights the potential involvement of ixodid ticks in the epidemiology of trypanosomes, as well as the use of tick cell lines for isolation of such tick-borne protozoa. Further studies are required to investigate the epidemiology, transmission and life cycle of this putative novel species.
从医学和兽医的角度来看，锥虫长期以来一直被认为是最重要的脊椎动物原生动物寄生虫之一。虽然已经确定了许多昆虫种类作为传播媒介，但蜱的作用还不太清楚。在这里，我们报告了从斯洛伐克采集的游离伊蚊蜱中分离和部分分子鉴定一种新型锥虫。该锥虫在蜱细胞培养中分离出来，然后通过显微镜观察和扩增 18S rRNA 和 24Sα rDNA 基因的片段进行部分鉴定。对所得序列的分析表明，被指定为布拉迪斯拉发锥虫 1 的锥虫可能是一种新物种，与从南美的蜱或亚洲的蜱中分离或检测到的几种或几种锥虫以及从巴西的犬中分离的犬锥虫密切相关。本研究强调了蜱在锥虫流行病学中的潜在作用，以及使用蜱细胞系分离此类蜱传原生动物的可能性。需要进一步研究以调查这种假定的新型物种的流行病学、传播和生命周期。
