School of Pharmacy, Eastern Asia University, Pathum Thani, 12110, Thailand.
Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand.
J Antibiot (Tokyo). 2021 Jan;74(1):51-58. doi: 10.1038/s41429-020-0353-y. Epub 2020 Jul 28.
A novel actinomycete strain CYP1-5 was isolated from the mountain soil sample collected from Chaiyaphum province, Thailand and its taxonomic position was clarified by using a polyphasic taxonomic approach. The chemotaxonomic properties of strain CYP1-5 were consistent within the genus Actinomadura. Cell-wall peptidoglycan of this strain contained meso-diaminopimelic acid. Galactose, madurose, and ribose were presented as the diagnostic sugars in whole-cell hydrolysates. The major menaquinone was MK-9(H). Major cellular fatty acids were iso-C and C. Phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, and phosphatidylinositol mannoside were observed as predominant phospholipids. Based on the results of phylogenetic analyses of 16S rRNA gene sequence, strain CYP1-5 was constituent with the genus Actinomadura and was closely related to Actinomadura syzygii GKU157 (99.5%) and Actinomadura chibensis IFM 10266 (= JCM 14158) (98.2%). The draft genome size of strain CYP1-5 was 9.30 Mb with 72.2 mol% of G + C content. Strain CYP1-5 showed ANIb values of 94.9% with A. syzygii GKU157 and 93.2% with A. chibensis JCM 14158. Phenotypic characteristics, phylogenetic analysis and genome data support that strain CYP1-5 could be discriminated from its closest relatives, representing a novel species of the genus Actinomadura, for which the name Actinomadura decatromicini sp. nov. is proposed. The type strain is CYP1-5 (= JCM 16996 = KCTC 19916 = TISTR 2901).
一株新型放线菌 CYP1-5 菌株从泰国猜也蓬府采集的山地土壤样本中分离得到，并通过多相分类学方法阐明了其分类地位。该菌株的化学分类特征与链霉菌属内的其他种一致。细胞壁肽聚糖含有 meso-二氨基庚二酸。全细胞水解物中含有半乳糖、马杜糖和核糖，这些糖可作为该菌的特征性糖。主要的menaquinone 为 MK-9(H)。主要的细胞脂肪酸为 iso-C 和 C。在磷脂分析中，发现了磷脂酰甘油、双磷脂酰甘油、磷脂酰肌醇和磷脂酰肌醇甘露糖苷。基于 16S rRNA 基因序列的系统发育分析结果，菌株 CYP1-5 与链霉菌属的其他种聚为一支，与 Actinomadura syzygii GKU157（99.5%）和 Actinomadura chibensis IFM 10266（= JCM 14158）（98.2%）的亲缘关系最近。CYP1-5 菌株的基因组草图大小为 9.30 Mb，G+C 含量为 72.2 mol%。菌株 CYP1-5 与 A. syzygii GKU157 的 ANIb 值为 94.9%，与 A. chibensis JCM 14158 的 ANIb 值为 93.2%。表型特征、系统发育分析和基因组数据支持 CYP1-5 菌株可以与其最接近的亲缘种区分开来，代表了链霉菌属的一个新种，命名为 Actinomadura decatromicini sp. nov.。该模式菌株为 CYP1-5（=JCM 16996=KCTC 19916=TISTR 2901）。
