Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung, 402, Taiwan.
Department of Veterinary Medicine, National Chiayi University, Chiayi, Taiwan.
Sci Rep. 2020 Jul 29;10(1):12686. doi: 10.1038/s41598-020-69686-5.
Despite great advancement in genetic typing, phenotyping is still an indispensable tool for categorization of bacteria. Certain amino acids may be essential for bacterial survival, growth, pathogenicity or toxin production, which prompts the idea that the intrinsic ability to utilize single amino acid under live-or-die situation could be a basis for differentiation of bacteria species. In this study, we determined the single amino acid consumption profiles of 7 bacterial species, and demonstrated that most bacteria have species-specific pattern of amino acid consumption. We also discovered that bacterial strains from different hosts, toxigenicity, and antibiotic-resistance presented distinct preference for certain amino acids. Taken altogether, the amino acid consumption profiles showed potential to be a novel tool complementary to study not only bacterial categorization but also biochemical characteristics of the bacteria such that its phenotyping can be used to uncover strategies for nutritional, pharmaceutical, taxonomic, and evolutionary aspects of bacterial researches.
尽管基因分型取得了巨大进展，但表型分析仍然是细菌分类的不可或缺的工具。某些氨基酸可能对细菌的生存、生长、致病性或毒素产生至关重要，这促使人们认为在生死攸关的情况下利用单一氨基酸的内在能力可能是区分细菌物种的基础。在这项研究中，我们确定了 7 种细菌的单一氨基酸消耗谱，并证明大多数细菌都具有特定的氨基酸消耗模式。我们还发现，来自不同宿主、产毒性和抗药性的细菌菌株对某些氨基酸表现出明显的偏好。总的来说，氨基酸消耗谱有可能成为一种新的工具，不仅可以补充细菌分类的研究，还可以研究细菌的生化特性，因此可以通过表型分析来揭示细菌在营养、药物、分类和进化方面的研究策略。
