Langlois Bruce E, Parlindungan Abdul Karim, Harmon Robert J, Akers Katherine
Department of Animal Sciences, University of Kentucky, Lexington, KY 40546-0215.
J Food Prot. 1990 Feb;53(2):119-126. doi: 10.4315/0362-028X-53.2.119.
Thirty-three biochemical characteristics were compared for 855 staphylococci isolated from human (93) and bovine sources (762). Differences in the predominant Staphylococcus species present and biochemical characteristics of species were observed for isolates from bovine milk and human clinical sources as well as between milk isolates obtained during the 1960s and 1980s from the same herd. All isolates were bacitracin-resistant and 98% were lysostaphin-sensitive. Approximately 31% of S. hyicus strains were coagulase-positive. Thermonuclease activity was observed for strains other than S. aureus . Milk strains were more salt tolerant than human strains. Carbohydrates tended to be utilized by a greater percentage of milk strains than human strains within a given species. Many milk isolates failed to utilize glycerol in the presence of 0.4 μg erythromycin/mL. The results of this study indicate that the source of isolates may influence the predominant species present and species biochemical characteristics. These differences in species characteristics may affect results obtained for identification of nonhuman isolates with rapid identification systems where the data bases are generally based on human clinical isolates.
对从人类（93株）和牛源（762株）分离出的855株葡萄球菌的33种生化特性进行了比较。观察到来自牛乳和人类临床源的分离株以及20世纪60年代和80年代从同一牛群获得的乳源分离株在主要葡萄球菌种类和菌种生化特性方面存在差异。所有分离株对杆菌肽耐药，98%对溶葡萄球菌素敏感。约31%的猪葡萄球菌菌株凝固酶阳性。除金黄色葡萄球菌外的其他菌株观察到有耐热核酸酶活性。乳源菌株比人类菌株更耐盐。在给定菌种中，利用碳水化合物的乳源菌株百分比往往高于人类菌株。许多乳源分离株在存在0.4μg/mL红霉素的情况下无法利用甘油。本研究结果表明，分离株的来源可能影响主要存在的菌种和菌种生化特性。这些菌种特性的差异可能会影响使用快速鉴定系统鉴定非人源分离株的结果，因为这些系统的数据库通常基于人类临床分离株。
