作为生物偶联平台的 RAFT 聚合物的杂 Diels-Alder 环加成反应。

Institute of Biological and Chemical Systems (IBCS), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.

Macromolecular Architectures, Institute for Chemical Technology and Polymer Chemistry (ITCP), Karlsruhe Institute of Technology (KIT), Engesserstr. 18, 76131, Karlsruhe, Germany.

Angew Chem Int Ed Engl. 2020 Nov 2;59(45):19951-19955. doi: 10.1002/anie.202005747. Epub 2020 Sep 2.

We introduce the bioconjugation of polymers synthesized by RAFT polymerization, bearing no specific functional end group, by means of hetero-Diels-Alder cycloaddition through their inherent terminal thiocarbonylthio moiety with a diene-modified model protein. Quantitative conjugation occurs over the course of a few hours, at ambient temperature and neutral pH, and in the absence of any catalyst. Our technology platform affords thermoresponsive bioconjugates, whose aggregation is solely controlled by the polymer chains.

我们通过其固有末端硫羰基硫基团与二烯改性模型蛋白的杂狄尔斯-阿尔德环加成反应，实现了通过 RAFT 聚合合成的、不带有特定功能端基的聚合物的生物偶联。在几小时内、在环境温度和中性 pH 值以及没有任何催化剂的情况下，即可实现定量偶联。我们的技术平台提供了热响应性生物偶联物，其聚集仅受聚合物链控制。