Fan Tingting, Ren Rui, Tang Shaojun, Zhou Yiyun, Cai Meng, Zhao Wenwen, He Yuelin, Xu Jun
The Laboratory of Forestry Genetics, Central South University of Forestry and Technology, Changsha, China.
The Center of Culture Preservation, Hunan Institute of Microbiology, Changsha, China.
Front Microbiol. 2023 Feb 1;14:1079353. doi: 10.3389/fmicb.2023.1079353. eCollection 2023.
Morels () are one of the most popular edible fungi in the world, especially known for their rich nutrition and delicious taste. Earlier research indicates that the production of fruiting bodies can be affected by the growth of mycelium. To investigate the molecular mechanisms underlying mycelium growth in , we performed transcriptome analysis and metabolomics analysis of three growth stages of the hypha of . As a result, 24 differentially expressed genes, such as transketolase (tktA), glucose-6-phosphate dehydrogenase (G6PDH), fructose-diphosphate aldolase (Fba), and ribose-5-phosphate isomerase (rpiA), as well as 15 differentially accumulated metabolites, including succinate and oxaloacetate, were identified and considered as the key genes and metabolites to mycelium growth in . In addition, guanosine 3',5'-cyclic monophosphate (cGMP), guanosine-5'-monophosphate (GMP), and several small peptides were found to differentially accumulate in different growth stages. Furthermore, five pathways, namely, starch and sucrose metabolism, pentose and glucuronate interconversions, fructose and mannose metabolism, tyrosine metabolism, and purine nucleotides, enriched by most DEGs, existed in the three compared groups and were also recognized as important pathways for the development of mycelium in morels. The comprehensive transcriptomics and metabolomics data generated in our study provided valuable information for understanding the mycelium growth of , and these data also unveiled the key genes, metabolites, and pathways involved in mycelium growth. This research provides a great theoretical basis for the stable production and breeding of morels.
羊肚菌是世界上最受欢迎的可食用真菌之一，尤其以其丰富的营养和美味的口感而闻名。早期研究表明，子实体的产生会受到菌丝体生长的影响。为了研究羊肚菌菌丝体生长的分子机制，我们对羊肚菌菌丝体的三个生长阶段进行了转录组分析和代谢组分析。结果，鉴定出24个差异表达基因，如转酮醇酶（tktA）、葡萄糖-6-磷酸脱氢酶（G6PDH）、果糖二磷酸醛缩酶（Fba）和核糖-5-磷酸异构酶（rpiA），以及15种差异积累的代谢物，包括琥珀酸和草酰乙酸，它们被认为是羊肚菌菌丝体生长的关键基因和代谢物。此外，还发现环磷酸鸟苷（cGMP）、鸟苷-5'-单磷酸（GMP）和几种小肽在不同生长阶段有差异积累。此外，淀粉和蔗糖代谢、戊糖和葡糖醛酸相互转化、果糖和甘露糖代谢、酪氨酸代谢以及嘌呤核苷酸这五条途径在三个比较组中被大多数差异表达基因富集，也被认为是羊肚菌菌丝体发育的重要途径。我们研究中产生的综合转录组学和代谢组学数据为理解羊肚菌的菌丝体生长提供了有价值的信息，这些数据还揭示了参与菌丝体生长的关键基因、代谢物和途径。这项研究为羊肚菌的稳定生产和育种提供了重要的理论基础。
