Nakashima Kazuki, Ishida Aiko, Shimamoto Saki, Ijiri Daichi, Ohtsuka Akira
Division of Animal Metabolism and Nutrition, Institute of Livestock and Grassland Science, NARO, Tsukuba 305-0901, Japan.
Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University, Kagoshima 890-0065, Japan.
J Poult Sci. 2020 Jul 25;57(3):205-209. doi: 10.2141/jpsa.0190082.
Insulin stimulates protein synthesis in skeletal muscles. Protein synthesis is controlled by the mechanistic target of rapamycin (mTOR) signaling in skeletal muscles. This study was conducted to investigate the effect of insulin on protein synthesis and mTOR signaling in chick myotube cultures. Chick myotubes were incubated with insulin (1 g/m) for 1 h. Protein synthesis, measured using the surface sensing of translation method, was significantly increased by insulin. The phosphorylation of AKT (Thr308 and Ser473), p70 ribosomal S6 kinase 1 (S6K1, Thr389), S6 ribosomal protein (Ser235/236), and eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1, Thr37/46) was also significantly increased by insulin. These results suggest that insulin stimulates protein synthesis via mTOR signaling (phosphorylation of AKT, S6K1, S6 ribosomal protein, and 4E-BP1) in chick myotube cultures.
胰岛素刺激骨骼肌中的蛋白质合成。蛋白质合成受骨骼肌中雷帕霉素机制性靶标（mTOR）信号传导的控制。本研究旨在探讨胰岛素对鸡肌管培养物中蛋白质合成和mTOR信号传导的影响。将鸡肌管用胰岛素（1μg/ml）孵育1小时。使用翻译表面传感法测量，胰岛素显著增加了蛋白质合成。胰岛素还显著增加了AKT（苏氨酸308和丝氨酸473）、p70核糖体S6激酶1（S6K1，苏氨酸389）、S6核糖体蛋白（丝氨酸235/236）和真核翻译起始因子4E结合蛋白1（4E-BP1，苏氨酸37/46）的磷酸化。这些结果表明，在鸡肌管培养物中，胰岛素通过mTOR信号传导（AKT、S6K1、S6核糖体蛋白和4E-BP1的磷酸化）刺激蛋白质合成。
