Chen Eric C H, Mathieu Stephanie, Hoffrichter Anne, Ropars Jeanne, Dreissig Steven, Fuchs Jörg, Brachmann Andreas, Corradi Nicolas
Department of Biology, University of Ottawa, Ottawa, ON, Canada.
Genetics, Biocenter, LMU Munich, Martinsried, Germany.
Front Plant Sci. 2020 Jul 7;11:912. doi: 10.3389/fpls.2020.00912. eCollection 2020.
Evidence for the existence of dikaryote-like strains, low nuclear sequence diversity and inter-nuclear recombination in arbuscular mycorrhizal fungi has been recently reported based on single nucleus sequencing data. Here, we aimed to support evidence of inter-nuclear recombination using an approach that filters SNP calls more conservatively, keeping only positions that are exclusively single copy and homozygous, and with at least five reads supporting a given SNP. This methodology recovers hundreds of putative inter-nucleus recombination events across publicly available sequence data from individual nuclei. Challenges related to the acquisition and analysis of sequence data from individual nuclei are highlighted and discussed, and ways to address these issues in future studies are presented.
最近基于单核测序数据报道了丛枝菌根真菌中存在类双核体菌株、低核序列多样性和核间重组的证据。在此，我们旨在使用一种更保守地过滤单核苷酸多态性（SNP）调用的方法来支持核间重组的证据，只保留那些完全是单拷贝且纯合的位置，并且至少有五条读数支持给定的SNP。这种方法从来自单个细胞核的公开可用序列数据中发现了数百个推定的核间重组事件。文中强调并讨论了与从单个细胞核获取和分析序列数据相关的挑战，并提出了在未来研究中解决这些问题的方法。
