Zarei Mina, Jafarian Amir Hossein, Harandi Azadeh, Javidi Maryam, Gharechahi Maryam
Dental Research Center, Department of Endodontics, Mashhad Dental School, Mashhad University of Medical Science, Mashhad, Iran.
Pathology Research Center, Mashhad University of Medical Science, Mashhad, Iran.
Iran J Basic Med Sci. 2017 Feb;20(2):172-177. doi: 10.22038/ijbms.2017.8243.
The purpose of the present study was the immunohistochemical evaluation of VEGF and VII factors in dog's teeth pulp revascularized with MTA and propolis.
144 mature and immature two rooted dog's premolar canals were selected. Pulp necrosis and infection were established after 2 weeks and the disinfection of the canals was done with copious NaOCl irrigation and triantibiotic mixture (ciprofloxacin, metronidazole, and minocycline) for 3 weeks. Subsequently, the blood clot was evoked in the canal by periapical tissue irritation with a k-file. The samples were randomly divided into 6 experimental groups: propolis (groups 1, 2), MTA (groups 3, 4), and parafilm (groups 5, 6) in immature and mature teeth. The animals were sacrificed and samples were prepared for immunohistochemical evaluation of VEGF and the VIII factor.
Tissue regeneration was seen in 64.5% of MTA, 38% of propolis, and 0% of parafilm group samples. Expression of VEGF and VIII factor in the propolis group was more than the MTA group and it showed a reduction after 3 months in comparison to 1 month. VEGF and VIII factor were seen in stromal cells in addition to endothelial vessel cells. Overall, expression of angiogenic factors was more in the open apex teeth compared to close apex ones.
According to the results of this study, propolis can induce the expression of VEGF and VIII factor in infected mature and immature dog's teeth and is a suitable biomaterial for the revascularization technique.
本研究旨在对用MTA和蜂胶使牙髓血管再生的犬牙牙髓进行VEGF和VII因子的免疫组化评估。
选取144颗成熟和未成熟的双根犬前磨牙根管。2周后造成牙髓坏死和感染，用大量次氯酸钠冲洗和三联抗生素混合物（环丙沙星、甲硝唑和米诺环素）对根管进行消毒3周。随后，用k锉刺激根尖周组织在根管内诱发血凝块。样本随机分为6个实验组：未成熟和成熟牙齿中的蜂胶组（第1、2组）、MTA组（第3、4组）和石蜡膜组（第5、6组）。处死动物并制备样本用于VEGF和VIII因子的免疫组化评估。
在MTA组样本中64.5%出现组织再生，蜂胶组为38%，石蜡膜组为0%。蜂胶组中VEGF和VIII因子的表达高于MTA组，与1个月时相比，3个月后表达降低。除内皮血管细胞外，在基质细胞中也可见VEGF和VIII因子。总体而言，开放根尖的牙齿中血管生成因子的表达比闭合根尖的牙齿更多。
根据本研究结果，蜂胶可诱导感染的成熟和未成熟犬牙中VEGF和VIII因子的表达，是牙髓血管再生技术的合适生物材料。
