Juniarti Devi E, Kunarti Sri, Mardiyah Andi A, Purniati Ni M D
Department of Conservative Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Specialist Program of Conservative Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Eur J Dent. 2023 Jul;17(3):706-712. doi: 10.1055/s-0042-1749155. Epub 2022 Jul 11.
This study aimed to prove that the effect of diode laser 650-nm irradiation to the expression of vascular endothelial growth factor (VEGF)-A and transforming growth factor (TGF)-β1 plays important roles in dental pulp-regulating cell proliferation, differentiation, and revascularization.
The research was performed by randomized posttest only control group design using . A total of 48 samples were provided and divided into eight groups of 6 samples each with a random-sample allocation. Each group were prepared, and perforation of maxillary first molar were done. In control groups (groups 1-4), glass ionomer cement (GIC) was used to restore the teeth, while in laser groups (groups 5-8), the teeth were irradiated with diode laser 650 nm for 40 seconds before application of GIC. Half of the groups (groups 1, 2, 5, and 6) were necropsied in 7 days, and the rest (groups 3, 4, 7, and 8) were necropsied in 14 days. Immunohistochemistry (IHC) evaluation were implemented to check the expression of both VEGF-A and TGF-β1.
Both data of VEGF-A and TGF-β1 expression were analyzed using a one-way ANOVA ( = 0.05) with SPSS statistical software.
The study showed that the diode laser 650-nm irradiation increased expression of VEGF-A and TGF-β1, and there was a significant difference between diode laser and control group on VEGF-A expression ( = 0.001) and TGF- β1 ( = 0.000) on days 7 and 14.
Diode laser 650 nm with 40-second irradiation time shows increment from day 7 to day 14 reflecting increase in pulp healing by modulating VEGF-A and TGF-β1 expression since days 7 to 14.
本研究旨在证明650纳米二极管激光照射对血管内皮生长因子（VEGF）-A和转化生长因子（TGF）-β1表达的影响在调节牙髓细胞增殖、分化和血管再生中起重要作用。
本研究采用仅后测随机对照组设计进行。共提供48个样本，随机分为8组，每组6个样本。对每组样本进行制备，并对上颌第一磨牙进行穿孔。在对照组（1-4组）中，使用玻璃离子水门汀（GIC）修复牙齿，而在激光组（5-8组）中，在应用GIC之前，用650纳米二极管激光照射牙齿40秒。一半的组（1、2、5和6组）在7天后进行尸检，其余组（3、4、7和8组）在14天后进行尸检。采用免疫组织化学（IHC）评估来检测VEGF-A和TGF-β1的表达。
使用SPSS统计软件，对VEGF-A和TGF-β1表达的数据进行单因素方差分析（α = 0.05）。
研究表明，650纳米二极管激光照射可增加VEGF-A和TGF-β1的表达，在第7天和第14天，二极管激光组与对照组在VEGF-A表达（P = 0.001）和TGF-β1表达（P = 0.000）上存在显著差异。
照射时间为40秒的650纳米二极管激光从第7天到第14天显示出增加，这反映出从第7天到第14天通过调节VEGF-A和TGF-β1的表达促进了牙髓愈合。
