miR-1258 通过靶向 E2F1 调控细胞增殖和细胞周期抑制乳腺癌进展。

miR-1258 Regulates Cell Proliferation and Cell Cycle to Inhibit the Progression of Breast Cancer by Targeting E2F1.

机构信息

Department of Oncology, Yiwu Central Hospital, No. 699 Nanmen Road, 322000 Yiwu, China.

出版信息

Biomed Res Int. 2020 Jul 15;2020:1480819. doi: 10.1155/2020/1480819. eCollection 2020.

OBJECTIVE

This study is designed to clarify that miR-1258 targets E2F1 to regulate the proliferation and cell cycle of breast cancer (BC) cells and consequently suppress the progression of BC.

METHODS

Bioinformatics analysis was used to analyze the differentially expressed genes in BC. The expression of miR-1258 and E2F1 mRNA in BC cell lines and immortalized breast epithelial cell lines were detected by qRT-PCR. The proliferation and growth activity of BC cells were detected by MTT and colony formation assays. The apoptosis and cell cycle of BC cells were detected by flow cytometry and the targeting relationship between miR-1258 and E2F1 was identified by dual-luciferase assay.

RESULTS

The expression of miR-1258 was decreased while that of E2F1 was increased in BC cells. Overexpression of miR-1258 and silencing E2F1 could inhibit the cell proliferation and growth, block cells in the G0/G1 phase, and promote cell apoptosis. Besides, miR-1258 inhibited cell proliferation and growth, block cells in the G0/G1 phase, and promote cell apoptosis by downregulating E2F1.

CONCLUSION

miR-1258 regulates the proliferation and cell cycle to inhibit the progression of BC by targeting and downregulating E2F1.

目的

本研究旨在阐明 miR-1258 通过靶向 E2F1 来调节乳腺癌（BC）细胞的增殖和细胞周期，从而抑制 BC 的进展。

方法

利用生物信息学分析方法分析 BC 中的差异表达基因。通过 qRT-PCR 检测 BC 细胞系和永生化乳腺上皮细胞系中 miR-1258 和 E2F1 mRNA 的表达。通过 MTT 和集落形成实验检测 BC 细胞的增殖和生长活性。通过流式细胞术检测 BC 细胞的凋亡和细胞周期，并通过双荧光素酶报告实验鉴定 miR-1258 与 E2F1 之间的靶向关系。