Silva Marta G, Madsen-Bouterse Sally A, Dassanayake Rohana P, Mousel Michelle, Knowles Donald P
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, United States.
Paul G. Allen School for Global Animal Health, Washington State University, Pullman, WA 99164-7040, United States.
Vet Anim Sci. 2019 Apr 6;7:100056. doi: 10.1016/j.vas.2019.100056. eCollection 2019 Jun.
Tissue inhibitor of metalloproteinase-1 (TIMP-1) and interleukin-10 (IL-10) were identified as potential biomarkers for ovine scrapie in a mouse model. The development of novel diagnostic methods to identify pre-clinical scrapie-infected animals is needed. In this study, ELISA was used to assess TIMP-1 and IL-10 levels in 158 serum samples from naïve and preclinical scrapie-infected sheep. Young (≤ 18 months) naïve sheep had significantly lower TIMP-1 levels compared with old (≥ 20 months) naïve and old infected sheep (<0.04). Young naïve sheep had lower IL-10 than old naïve sheep (<0.001). Both cytokines tended to have lower levels in young naïve sheep compared to infected sheep but this did not reach significance. A larger sample size will be helpful in determining the potential of these cytokines as a diagnostic tool.
金属蛋白酶组织抑制剂-1（TIMP-1）和白细胞介素-10（IL-10）被确定为小鼠模型中绵羊瘙痒病的潜在生物标志物。需要开发新的诊断方法来识别临床前感染瘙痒病的动物。在本研究中，酶联免疫吸附测定（ELISA）用于评估158份来自未感染和临床前感染瘙痒病绵羊的血清样本中的TIMP-1和IL-10水平。年轻（≤18个月）未感染绵羊的TIMP-1水平显著低于年老（≥20个月）未感染绵羊和年老感染绵羊（<0.04）。年轻未感染绵羊的IL-10水平低于年老未感染绵羊（<0.001）。与感染绵羊相比，年轻未感染绵羊的两种细胞因子水平均倾向于较低，但这未达到显著水平。更大的样本量将有助于确定这些细胞因子作为诊断工具的潜力。
